Invitrogen

cIAP1 Monoclonal Antibody (GT422)

Product Image Gallery

cIAP1 Antibody in Immunocytochemistry (ICC) — Immunofluorescence analysis of cIAP1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with cIAP1 Mouse Monoclonal Antibody (GT422) (Product # MA5-27728) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasm and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

cIAP1 Antibody in Western Blot (WB) — Western blot was performed using Anti-cIAP1 Monoclonal Antibody (GT422) (Product # MA5-27728) and a 64 kDa corresponding to BIRC2 along with an uncharacterized band (*) around 45 kDa was observed across cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), HCT 116 (Lane 3), MCF-7 (Lane 4), MDA-MB-231 (Lane 5) and Jurkat (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Western Blot (WB)

1:500-1:3,000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

GT422

Immunogen

Recombinant protein encompassing a sequence within the center region of human cIAP1. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2735147

Product Specific Information

Positive Control: Jurkat, Raji, NCI-H929

Predicted Reactivity: Mouse (85%), Rat (80%), Dog (89%), Rhesus Monkey (98%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: API1; apoptosis inhibitor 1; baculoviral IAP repeat containing 2; baculoviral IAP repeat-containing 2; Baculoviral IAP repeat-containing protein 2; BIRC2; C-IAP1; Cellular inhibitor of apoptosis 1; Hiap-2; HIAP2; IAP homolog B; IAP-2; Inhibitor of apoptosis protein 2; MIHB; NFR2-TRAF signalling complex protein; RING finger protein 48; RING-type E3 ubiquitin transferase BIRC2; RNF48; TNFR2-TRAF-signaling complex protein 2

Gene Aliases: API1; BIRC2; c-IAP1; cIAP1; Hiap-2; HIAP2; MIHB; RNF48

UniProt ID: (Human) Q13490

Entrez Gene ID: (Human) 329

Molecular Function: enzyme modulator protease inhibitor