Invitrogen

cIAP1 Monoclonal Antibody (GT422)

Product Image Gallery

cIAP1 Antibody in Western Blot (WB) — Knockdown of BIRC2 was achieved by transfecting HeLa with BIRC2 specific siRNAs (Silencer® select Product # s1450). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the BIRC2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with cIAP1 Monoclonal Antibody (GT422) (Product # MA5-27728, 1:1000 dilution) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to BIRC2. An uncharacterized band was also observed at ~45 kDa.

cIAP1 Antibody in Immunocytochemistry (ICC) — Immunocytochemistry-Immunofluorescence analysis of cIAP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: cIAP1 Monoclonal Antibody (GT422) (Product # MA5-27728) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

GT422

Immunogen

Recombinant protein encompassing a sequence within the center region of human cIAP1. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2735147

Product Specific Information

Positive Control: Jurkat, Raji, NCI-H929

Predicted Reactivity: Mouse (85%), Rat (80%), Dog (89%), Rhesus Monkey (98%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: apoptosis inhibitor 1; baculoviral IAP repeat-containing 2; Baculoviral IAP repeat-containing protein 2; C-IAP1; Cellular inhibitor of apoptosis 1; hIAP-2; IAP homolog B; IAP-2; Inhibitor of apoptosis protein 2; NFR2-TRAF signalling complex protein; RING finger protein 48; RING-type E3 ubiquitin transferase BIRC2; TNFR2-TRAF-signaling complex protein 2

Gene Aliases: API1; BIRC2; c-IAP1; cIAP1; Hiap-2; HIAP2; MIHB; RNF48

UniProt ID: (Human) Q13490

Entrez Gene ID: (Human) 329

Molecular Function: enzyme modulator protease inhibitor