|Tested species reactivity||Bovine, Dog, Chicken, Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant N-terminal fragment of murine delta 1 Catenin.|
|Storage buffer||tissue culture supernatant|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
MA1-25157 detects delta 1 Catenin from rat, bovine, chicken, canine, human, and mouse samples.
MA1-25157 has been successfully used in ELISA, immunofluorescence, immunoprecipitation, immunocytochemistry and Western blot procedures. MA1-25157 detects a band ~120 kDa and a possible doublet band of ~200 kDa.
The MA1-25157 immunogen is recombinant N-terminal fragment of mouse delta 1 Catenin.
delta 1 Catenin is an efficient tyrosine kinase substrate implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Pou5f1-dependent EGF expression controls E-cadherin endocytosis, cell adhesion, and zebrafish epiboly movements.
MA1-25157 was used in immunohistochemistry - paraffin section to study the role of E-cadherin endosomal trafficking to development of zebrafish
|Song S,Eckerle S,Onichtchouk D,Marrs JA,Nitschke R,Driever W||Developmental cell (24:486)||2013|