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Immunofluorescent analysis of eGFP using either natural fluorescence (green) or an anti-eGFP antibody (red) in U2OS cells transfected with an EGFR-eGFP fusion protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were probed with a GFP Epitope Tag monoclonal antibody (Product # MA1-952) at a dilution of 1:20 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 550 goat anti-mouse IgG secondary antibody (Product # 84540) at a dilution of 1:200 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan Instrument at 20X magnification.
|Tested species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Full length enhanced green fluorescent protein (eGFP)|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:20 - 1:100|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-952 contains 100 ug of in vitro produced, Protein A purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide.
MA1-952 detects eGFP and eGFP tagged proteins.
MA1-952 has been successfully used in Western blot and immunofluorescence applications.
The MA1-952 immunogen is full length enhanced green fluorescent protein (eGFP).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cx31.1 acts as a tumour suppressor in non-small cell lung cancer (NSCLC) cell lines through inhibition of cell proliferation and metastasis.
MA1-952 was used in western blot to examine the role of Cx31.1 in non-small cell lung cancer
|Zhang D,Chen C,Li Y,Fu X,Xie Y,Li Y,Huang Y||Journal of cellular and molecular medicine (16:1047)||2012|