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Immunofluorescent analysis of eGFP using either natural fluorescence (green) or an eGFP antibody (red) in U2OS cells transfected with an EGFR-eGFP fusion protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were probed with an eGFP polyclonal antibody (Product # CAB4211) at a dilution of 1:20 for at least 1 hour at 37C, washed with PBS, and incubated with DyLight 550 goat anti-rabbit IgG secondary antibody (Product # 84541) at a dilution of 1:200 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan Instrument at 20X magnification.
|Tested species reactivity||Tag|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified recombinant eGFP|
|Purification||Antigen affinity chromatography|
|Storage buffer||BBS, pH 8.4|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CAB4211 detects eGFP in samples.
CAB4211 has been successfully used in Western Blot and ICC/IF applications.
The CAB4211 immunogen is purified recombinant eGFP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
enhanced GFP; Enhanced Green Fluorescence Protein; Venus