|Tested species reactivity||Tag|
|Host / Isotype||Sheep / IgG|
|Immunogen||A synthetic peptide|
|Purification||Ammonium sulfate precipitation|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||10-50 µg/ml|
|Western Blot (WB)||10-50 µg/ml|
Glycerol (1:1) may be added for additional stability.
Reconstitute with 500 ul of distilled water.
Centrifuge to remove any insoluble material.
GFP is a naturally fluorescent protein, discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin's blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
enhanced GFP; Enhanced Green Fluorescence Protein; Venus