Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human brain tissue stained using a eIF3A polyclonal antibody (Product # PA5-23285) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (563 KEHQRILARRQTIEERKE 580) of eIF3A protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||0.25-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
eIF3A is an important protein involved in protein translation/synthesis. It is a 1382 amino acid member of the eIF-3 subunit A family with a PCI domain and a component of the eIF-3 multiprotein complex composed of at least 13 different subunits. It interacts with eIF3B and intermediate filament protein K7 (cytokeratin 7). A function of eIF3A is to bind to the 40S ribosome and promote the binding of methionyl-tRNAi and mRNA and thus stabilize the translation initiation complex. This protein synthesis factor may also act as an adapter molecule between the translation initiation apparatus and the cytoskeleton structure in the cell. eIF3A may be required for cell division and is also involved in breast, cervical, esophageal and stomach carcicomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.