Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human brain tissue stained using a eIF3B polyclonal antibody (Product # PA5-23278) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (3 DAENVAVPEAAEERAEP 19) of eIF3B protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||0.05-0.25 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
eIF3B is an acidic protein belonging to the EIF-3 subunit B family with an RRM (RNA recognition motif) domain. An important protein involved in protein translation/synthesis, eIF3B is known to bind to the 40S ribosomal subunit and promotes the binding of methionyl-tRNAi and mRNA. eIF3B is a component of the EIF3 multiprotein complex, composed of at least 13 different subunits. It is also known to interact with eIF3A and directly with the p170 subunit of EIF3, the region required for this association is located within the RRM. Ubiquitous expression of eIF3B is seen in most of the tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.