Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human kidney tissue stained using a eIF3G polyclonal antibody (Product # PA5-23319) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Bovine, Dog, Horse, Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (210 TGKYVPPSLRDGASRRGE 227) of eIF3G protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||0.025-0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
eIF3G is an RNA-associated protein involved in protein synthesis. It is a member of the eIF-3 subunit G family and the largest of the eukaryotic translation initiation factor family with eukaryotic translation initiation factor-3 (eIF3) being a multiprotein complex composed of at least 13 different subunits. eIF3G has a RRM (RNA recognition motif) domain and is known to interact with eIF3K. The function of eIF3G is to bind to the 40S ribosomal subunit and promote the binding of methionyl-tRNAi and mRNA. This subunit of eukaryotic translation initiation factor-3 also binds to the 18S rRNA. It is ubiquitously expressed in most tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.