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eIF3e Antibody (MA5-49155) in WB
Western blot analysis of EIF3E using EIF3E antibody (Product # MA5-49155). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human Raji whole cell lysates; Lane 2: human K562 whole cell lysates; Lane 3: rat thymus tissue lysates; Lane 4: mouse thymus tissue lysates; Lane 5: mouse ANA-1 whole cell lysates; Lane 6: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were tr... View More
Product Details
MA5-49155
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
10F5H6
Immunogen
E.coli-derived human EIF3e recombinant protein (Position: A160-Q241).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074813
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human EIF3e shares 100% amino acid (aa) sequence identity with both mouse and rat EIF3e.
Target Information
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-3 p48; EIF3E; Eukaryotic translation initiation factor 3 subunit 6; Eukaryotic translation initiation factor 3 subunit E; eukaryotic translation initiation factor 3 subunit E isoform 2 transcript; eukaryotic translation initiation factor 3, subunit 6 (48kD); eukaryotic translation initiation factor 3, subunit 6 48kDa; eukaryotic translation initiation factor 3, subunit E; mammary tumor integration site 6; Mammary tumor-associated protein INT-6; mammary tumor-associated protein INT6; MMTV integration site 6; murine mammary tumor integration site 6 (oncogene homolog); Viral integration site protein INT-6; Viral integration site protein INT-6 homolog
Gene Aliases: 48kDa; eIF3-p46; EIF3-P48; EIF3E; EIF3S6; INT6
UniProt ID: (Human) P60228, (Mouse) P60229
Entrez Gene ID: (Human) 3646, (Mouse) 16341, (Rat) 103692906
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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