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eIF4A3 Antibody (MA5-41263) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of eIF4A3 in MCF-7 cells (green) using eIF4A3 Recombinant Monoclonal Antibody (Product # MA5-41263). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. The nuclear counter stain is DAPI (blue).
Product Details
MA5-41263
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JG35-33
Immunogen
Recombinant protein within N-terminal Human eIF4A3
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2899016
Target Information
eIF4A3 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. eIF4A3 is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; eIF-4A-III; eIF4A-III; Eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A, isoform 3; hNMP 265; NMP 265; Nuclear matrix protein 265
Gene Aliases: 2400003O03Rik; DDX48; eIF4A-III; EIF4A3; eIF4AIII; KIAA0111; mKIAA0111; MUK34; NMP265; NUK34; RCPS
UniProt ID: (Human) P38919, (Mouse) Q91VC3, (Rat) Q3B8Q2
Entrez Gene ID: (Human) 9775, (Mouse) 192170, (Rat) 688288
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
nuclear-transcribed mRNA poly(A) tail shortening
mRNA splicing, via spliceosome
rRNA processing
termination of RNA polymerase II transcription
RNA export from nucleus
mRNA export from nucleus
associative learning
RNA splicing
RNA secondary structure unwinding
response to organic cyclic compound
selenocysteine metabolic process
negative regulation of translation
mRNA 3'-end processing
exploration behavior
positive regulation of translation
embryonic cranial skeleton morphogenesis
cellular response to selenite ion
negative regulation of excitatory postsynaptic potential
negative regulation of selenocysteine incorporation
negative regulation of selenocysteine insertion sequence binding
cellular response to brain-derived neurotrophic factor stimulus
positive regulation of transcription from RNA polymerase II promoter
positive regulation of mRNA splicing, via spliceosome
regulation of alternative mRNA splicing, via spliceosome
mRNA processing
mRNA transport
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