|Tested species reactivity||Bovine|
|Published species reactivity||Rat, Non-human primate|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Full length bovine protein.|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 2 publications below|
MA1-25066 detects gamma Adaptin from bovine samples. MA1-25066 will not react with mouse or rat.
MA1-25066 has been successfully used in Western blot and immunoprecipitation procedures.
The MA1-25066 immunogen is full length bovine protein.
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Intracellular trafficking and activation of the furin proprotein convertase: localization to the TGN and recycling from the cell surface.
MA1-25066 was used in immunocytochemistry to investigate the dynamic distribution and functional properties of furin in cells
|Molloy SS,Thomas L,VanSlyke JK,Stenberg PE,Thomas G||The EMBO journal (13:18)||1994|
Targeting and mistargeting of plasma membrane adaptors in vitro.
MA1-25066 was used in immunocytochemistry to investigate the intracellular transport and sequestration of plasma membrane adaptors
|Seaman MN,Ball CL,Robinson MS||The Journal of cell biology (123:1093)||1993|