Immunofluorescent analysis of gamma Catenin in MDCK cells, fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Polyethylene glycol tert-octylphenyl ether, using a gamma Catenin monoclonal antibody (Product # MA1-25155) at a dilution of 1:500 followed by detection using a Goat anti-Mouse IgG, Atto-488 conjugate. Nuclei were counterstained with DAPI (blue).
|Tested species reactivity||Bovine, Dog, Chicken, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant, full length chicken protein.|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25155 detects gamma Catenin from human, bovine, chicken, and canine samples.
MA1-25155 has been successfully used in immunocytochemistry, immunohistochemistry (frozen), immunoprecipitation, and Western blot procedures.
The MA1-25155 immunogen is recombinant, full length chicken protein.
Gamma Catenin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of gamma Catenin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.