|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminus of human catenin-gamma protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is breast carcinoma.
The catenins (@alpha;, β, γ, and δ) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. α-catenin can also bind P-cadherin and N-cadherin. γ-catenin, also known aas plakoglobin, is an 82kDa protein that binds with a-catenin and N-cadherin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.