Immunofluorescent analysis of g-Tubulin using anti-g-Tubulin monoclonal antibody (Pierce Product# MA1-850, Clone 4D11) (shown in green) in HeLa (A) and U2-OS cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Pierce Product #37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing g-Tubulin (Pierce Product# MA1-850), at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Pierce Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DY-547 phalloidin, nuclei (blue) were stained with Hoechst 33342 dye (Pierce Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-850 detects gamma Tubulin from human, mouse, monkey, and rat samples.
MA1-850 has been successfully used in Western blot, immunoprecipitation, and immunofluorescence procedures.
The MA1-850 immunogen is a recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin.
Gamma Tubulin is required for structure and function in microtubule growing centers. Disruption of gamma Tubulin has also shown to cause mitotic arrest and a sharp decrease in microtubule quantity. This disruption also causes mutations in the microtubule organizing centers never seen in wild-type cells. gamma Tubulin is exclusively localized to the spindle poles in animal cells. Localization and timing of gamma Tubulin during cell cycle is thought to be highly regulated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Substrate stiffness effect and chromosome missegregation in hIPS cells.
MA1-850 was used in immunocytochemistry to discuss how culturing conditions affect the genomic stability of pluripotent stem cells
|Acevedo-Acevedo S,Crone WC||Journal of negative results in biomedicine (14:null)||2015|
Inhibitory effects of Cyperus digitatus extract on human platelet function in vitro.
MA1-850 was used in western blot to study the mechanisms of antiplatelet action of Cyperus digitatus
|Fuentes E,Forero-Doria O,Alarcón M,Palomo I||Platelets (26:764)||2015|
|Not Applicable||Not Cited||
Topiramate Protects Pericytes from Glucotoxicity: Role for Mitochondrial CA VA in Cerebromicrovascular Disease in Diabetes.
MA1-850 was used in western blot to study the role for mitochondrial CA VA in cerebromicrovascular disease in diabetes and protection of pericytes from glucotoxicity by topiramate
|Patrick P,Price TO,Diogo AL,Sheibani N,Banks WA,Shah GN||Journal of endocrinology and diabetes (2:null)||2015|
TDP-43 causes differential pathology in neuronal versus glial cells in the mouse brain.
MA1-850 was used in western blot to study the different pathological effects of mutant TDP-43 in murine neuronal and glial cells
|Yan S,Wang CE,Wei W,Gaertig MA,Lai L,Li S,Li XJ||Human molecular genetics (23:2678)||2014|
Synphilin-1 attenuates neuronal degeneration in the A53T alpha-synuclein transgenic mouse model.
MA1-850 was used in immunohistochemistry to investigate the neuroprotective effect of synphilin-1 in the A53T alpha-synuclein transgenic mouse model
|Smith WW,Liu Z,Liang Y,Masuda N,Swing DA,Jenkins NA,Copeland NG,Troncoso JC,Pletnikov M,Dawson TM,Martin LJ,Moran TH,Lee MK,Borchelt DR,Ross CA||Human molecular genetics (19:2087)||2010|