|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human tubulin gamma protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is colon carcinoma.
Tubulin gamma, an essential structural protein for cell growth, organizes microtubule arrays in the nucleus and cytoplasm. Tubulin gamma- depleted cells fail to form functional spindles and arrest during nuclear division. Tubulin gamma associates with spindle body components Spc97 and Spc98 to form the Tubulin gamma complex. The phosphrylation of Tubulin gamma at Tyr445 plays a regulatory role in microtubule formation. The incidence rate for this phosphorylation event peaks during G1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.