Invitrogen

gamma Tubulin Monoclonal Antibody (4D11)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

7 Published Figures

21 References

View all (28) gamma Tubulin antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

gamma Tubulin Antibody in Western Blot (WB)

FIGURE: 1 / 14

gamma Tubulin Antibody (MA1-850) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of A-549 (Lane 1), MCF-7 (Lane 2), HeLa (Lane 3), HepG2 (Lane 4), Caco2 (Lane 5), A-431 (Lane 6), SH-SY5Y (Lane 7) and U-2 OS (Lane 8). The blot was probed with Anti-gamma Tubulin Mouse Monoclonal Antibody (Product # MA1-850, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 51 kDa band corresponding to gamma Tubulin was observed across th... View More

Product Image Gallery

gamma Tubulin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of g-Tubulin using anti-g-Tubulin monoclonal antibody (Product # MA1-850, Clone 4D11) (shown in green) in HeLa (A) and U2-OS cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing g-Tubulin (Product # MA1-850), at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product # 35503) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DY-547 phalloidin, nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

gamma Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on cancer biopsies of deparaffinized human Breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing gamma Tubulin (Product # MA1-850) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

gamma Tubulin Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with gamma Tubulin specific validated siRNA (Silencer® select Product # S14502) and decrease in signal intensity was observed in western blot application using Anti-gamma Tubulin Mouse Monoclonal Antibody (Product # MA1-850). {KD}

Product Details

MA1-850

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

1:200

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:50

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Non-human primate, Rat, Human, Mouse

Published species

Fruit fly, Human, Mouse, Plant

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

4D11

Immunogen

Recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2211249

Product Specific Information

MA1-850 detects gamma Tubulin from human, mouse, monkey, and rat samples.

MA1-850 has been successfully used in Western blot, immunoprecipitation, and immunofluorescence procedures.

The MA1-850 immunogen is a recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin.

Target Information

This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Electrofusion Stimulation Is an Independent Factor of Chromosome Abnormality in Mice Oocytes Reconstructed via Spindle Transfer.

Frontiers in endocrinology

Wang W,Shao S,Chen W,Wang W,Chuai Y,Li Y,Guo Y,Han S,Shu M,Wang Q,Zhang L,Shang W

MA1-850 was used in Immunohistochemistry to compare the effects of enucleation and electrofusion stimulation on maturation promoting factor activity, spindle morphology, γ-tubulin localization and chromosome arrangement in mice oocytes.

Fri Feb 11 00:00:00 EST 2022

The histone demethylase KDM5 is required for synaptic structure and function at the Drosophila neuromuscular junction.

Cell reports

Belalcazar HM,Hendricks EL,Zamurrad S,Liebl FLW,Secombe J

MA1-850 was used in Western Blot to stud the role of the lysine demethylase 5 (KDM5) family of histone demethylases impacts transcriptional programs to mediate synaptic structure and activity.

Tue Feb 16 00:00:00 EST 2021

S-acylation by ZDHHC20 targets ORAI1 channels to lipid rafts for efficient Ca²⁺ signaling by Jurkat T cell receptors at the immune synapse.

eLife

Carreras-Sureda A,Abrami L,Ji-Hee K,Wang WA,Henry C,Frieden M,Didier M,van der Goot FG,Demaurex N

MA1-850 was used in Western Blotting to establish S-acylation as a critical regulator of ORAI1 channel trafficking and function at the IS and reveal that ORAI1 S-acylation enhances TCR recruitment to the synapse.

Thu Dec 16 00:00:00 EST 2021

S-acylation by ZDHHC20 targets ORAI1 channels to lipid rafts for efficient Ca²⁺ signaling by Jurkat T cell receptors at the immune synapse.

eLife

Carreras-Sureda A,Abrami L,Ji-Hee K,Wang WA,Henry C,Frieden M,Didier M,van der Goot FG,Demaurex N

Thu Dec 16 00:00:00 EST 2021

Contribution of synergism between PHF8 and HER2 signalling to breast cancer development and drug resistance.

EBioMedicine

Liu Q,Borcherding NC,Shao P,Maina PK,Zhang W,Qi HH

Published figure using gamma Tubulin monoclonal antibody (Product # MA1-850) in Western Blot

Wed Jan 01 00:00:00 EST 2020

Nucleoplasmic signals promote directed transmembrane protein import simultaneously via multiple channels of nuclear pores.

Nature communications

Mudumbi KC,Czapiewski R,Ruba A,Junod SL,Li Y,Luo W,Ngo C,Ospina V,Schirmer EC,Yang W

MA1-850 was used in Western Blotting to suggest that peripheral channel transport is the default mechanism that was adapted in evolution to include aspects of receptor-mediated central channel transport for directed trafficking of certain membrane proteins.

Mon May 04 00:00:00 EDT 2020

Topiramate Protects Pericytes from Glucotoxicity: Role for Mitochondrial CA VA in Cerebromicrovascular Disease in Diabetes.

Journal of endocrinology and diabetes

Patrick P,Price TO,Diogo AL,Sheibani N,Banks WA,Shah GN

MA1-850 was used in western blot to study the role for mitochondrial CA VA in cerebromicrovascular disease in diabetes and protection of pericytes from glucotoxicity by topiramate

Wed Sep 30 00:00:00 EDT 2020

Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells.

Oncology reports

Ohmine S,Salisbury JL,Ingle J,Pettinato G,Haddox CL,Haddad T,Galanis E,Ikeda Y,D'assoro AB

MA1-850 was used in Immunohistochemistry-Immunofluorescence to propose that Aurora-A-targeted therapy could represent a promising prophylactic therapeutic strategy to decrease the likelihood of CIN and development of aggressive teratomas derived from hiPSCs.

Sun Apr 01 00:00:00 EDT 2018

Cyclosporine A binding to COX-2 reveals a novel signaling pathway that activates the IRE1α unfolded protein response sensor.

Scientific reports

Groenendyk J,Paskevicius T,Urra H,Viricel C,Wang K,Barakat K,Hetz C,Kurgan L,Agellon LB,Michalak M

MA1-850 was used in Western Blotting to identify a novel and unanticipated cyclosporine-dependent signalling pathway, with cyclosporine triggering direct activation of the unfolded protein response.

Mon Nov 12 00:00:00 EST 2018

Phaseolus vulgaris Exerts an Inhibitory Effect on Platelet Aggregation through AKT Dependent Way.

Preventive nutrition and food science

Rodríguez-Azúa R,Quinteros EF,Olate-Briones A,Moore-Carrasco R

MA1-850 was used in Western Blotting to describe a novel property of the Phaseolus vulgaris: inhibition of thrombotic cardiovascular events.

Fri Jun 01 00:00:00 EDT 2018

Nucleoside Diphosphate Kinase-C Suppresses cAMP Formation in Human Heart Failure.

Circulation

Abu-Taha IH,Heijman J,Hippe HJ,Wolf NM,El-Armouche A,Nikolaev VO,Schäfer M,Würtz CM,Neef S,Voigt N,Baczkó I,Varró A,Müller M,Meder B,Katus HA,Spiger K,Vettel C,Lehmann LH,Backs J,Skolnik EY,Lutz S,Dobrev D,Wieland T

MA1850 was used in western blot to demonstrate that nucleoside diphosphate kinase-C suppresses cAMP formation in human heart failure

Tue Feb 28 00:00:00 EST 2017

Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis.

Scientific reports

Wang WA,Liu WX,Durnaoglu S,Lee SK,Lian J,Lehner R,Ahnn J,Agellon LB,Michalak M

Published figure using gamma Tubulin monoclonal antibody (Product # MA1-850) in Western Blot

Wed Jul 19 00:00:00 EDT 2017

c-MYC drives histone demethylase PHF8 during neuroendocrine differentiation and in castration-resistant prostate cancer.

Oncotarget

Maina PK,Shao P,Liu Q,Fazli L,Tyler S,Nasir M,Dong X,Qi HH

MA1850 was used in western blot to elucidate the regulation of PHF8 and KDM3A during neuroendocrine differentiation and castration-resistant prostate cancer

Tue Nov 15 00:00:00 EST 2016

The novel function of JADE1S in cytokinesis of epithelial cells.

Cell cycle (Georgetown, Tex.)

Siriwardana NS,Meyer RD,Panchenko MV

MA1-850 was used in Western Blotting to identify a role for JADE1S in regulation of cytokinesis and suggest its function in the Aurora B kinase-mediated cytokinesis checkpoint.

Wed Jun 22 00:00:00 EDT 2016

Inhibitory effects of Cyperus digitatus extract on human platelet function in vitro.

Platelets

Fuentes E,Forero-Doria O,Alarcón M,Palomo I

MA1-850 was used in western blot to study the mechanisms of antiplatelet action of Cyperus digitatus

Thu Jun 30 00:00:00 EDT 2016

Substrate stiffness effect and chromosome missegregation in hIPS cells.

Journal of negative results in biomedicine

Acevedo-Acevedo S,Crone WC

MA1-850 was used in immunocytochemistry to discuss how culturing conditions affect the genomic stability of pluripotent stem cells

Fri Dec 18 00:00:00 EST 2015

TDP-43 causes differential pathology in neuronal versus glial cells in the mouse brain.

Human molecular genetics

Yan S,Wang CE,Wei W,Gaertig MA,Lai L,Li S,Li XJ

MA1-850 was used in western blot to study the different pathological effects of mutant TDP-43 in murine neuronal and glial cells

Thu May 15 00:00:00 EDT 2014

Identification of synthetic lethality of PRKDC in MYC-dependent human cancers by pooled shRNA screening.

BMC cancer

Zhou Z,Patel M,Ng N,Hsieh MH,Orth AP,Walker JR,Batalov S,Harris JL,Liu J

Published figure using gamma Tubulin monoclonal antibody (Product # MA1-850) in Western Blot

Sat Dec 13 00:00:00 EST 2014

A novel role of Eruca sativa Mill. (rocket) extract: antiplatelet (NF-κB inhibition) and antithrombotic activities.

Nutrients

Fuentes E,Alarcón M,Fuentes M,Carrasco G,Palomo I

MA1-850 was used in Western Blotting to examine the antiplatelet and antithrombotic activities of Eruca sativa Mill.

Fri Dec 12 00:00:00 EST 2014

NLRP7, a nucleotide oligomerization domain-like receptor protein, is required for normal cytokine secretion and co-localizes with Golgi and the microtubule-organizing center.

The Journal of biological chemistry

Messaed C,Akoury E,Djuric U,Zeng J,Saleh M,Gilbert L,Seoud M,Qureshi S,Slim R

Published figure using gamma Tubulin monoclonal antibody (Product # MA1-850) in Immunocytochemistry

Fri Dec 16 00:00:00 EST 2011

NLRP7, a nucleotide oligomerization domain-like receptor protein, is required for normal cytokine secretion and co-localizes with Golgi and the microtubule-organizing center.

The Journal of biological chemistry

Messaed C,Akoury E,Djuric U,Zeng J,Saleh M,Gilbert L,Seoud M,Qureshi S,Slim R

Published figure using gamma Tubulin monoclonal antibody (Product # MA1-850) in Immunocytochemistry

Fri Dec 16 00:00:00 EST 2011

Synphilin-1 attenuates neuronal degeneration in the A53T alpha-synuclein transgenic mouse model.

Human molecular genetics

Smith WW,Liu Z,Liang Y,Masuda N,Swing DA,Jenkins NA,Copeland NG,Troncoso JC,Pletnikov M,Dawson TM,Martin LJ,Moran TH,Lee MK,Borchelt DR,Ross CA

MA1-850 was used in immunohistochemistry to investigate the neuroprotective effect of synphilin-1 in the A53T alpha-synuclein transgenic mouse model

Tue Jun 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: centrosome marker; Gamma-1-tubulin; Gamma-2-tubulin; Gamma-tubulin complex component 1; GCP-1; Tubulin gamma-1 chain; Tubulin gamma-2 chain; tubulin, gamma 1; tubulin, gamma 2; tubulin, gamma polypeptide

Gene Aliases: 1500010O08Rik; AI451582; AI503389; AI504772; CDCBM4; GCP-1; TUBG; TUBG1; TUBG2; TUBGCP1; Tubgl

UniProt ID: (Human) P23258, (Human) Q9NRH3, (Mouse) P83887, (Rat) P83888, (Mouse) Q8VCK3

Entrez Gene ID: (Human) 7283, (Human) 27175, (Mouse) 103733, (Rat) 252921, (Rat) 680991, (Mouse) 103768

Molecular Function: tubulin