Immunofluorescent analysis of glycine dehydrogenase (decarboxylating) precursor showing staining in the mitochondria of HepG2 cells. HepG2 cells were fixed in ice-cold MeOH for 5 min and stained using a glycine dehydrogenase (decarboxylating) precursor polyclonal antibody (Product # PA5-22102) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 442 and 723 of Glycine dehydrogenase (Uniprot ID#P23378)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-22102 targets glycine dehydrogenase (decarboxylating) precursor in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-22102 immunogen is a recombinant fragment corresponding to a region within amino acids 442 and 723 of Glycine dehydrogenase (Uniprot ID#P23378).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.