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Product Details
31850
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken
Published species
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified avidin
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
10mM HEPES, pH 7.5, with 0.15M NaCl, 1mg/mL BSA
Contains
0.08% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient
RRID
AB_228209
Product Specific Information
Product # 31850 is capable of binding to Avidin D by two mechanisms, either through the antigen binding sites in the Fab portion of the antibody or through multiple biotin residues covalently attached to the protein.
The measured activity of the biotinylated antibody is 99% by a solid phase IgG binding assay.
Reconstitute using 1 mL of distilled water; product may be stored for several weeks as an undiluted liquid. After dilution, do not use for more than one day. After one year, the reconstituted liquid product should be stored frozen in small undiluted aliquots, avoiding freeze/thaw cycles.
Target Information
Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. This protein contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons. Each subunit binds one molecule of biotin, and studies have shown that tryptophan and lysine are involved in the binding site for biotin. The sequencing of the subunit indicates it consists of 128 amino acids. Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions. Avidin is stable over a wide range of pH values and temperatures. Extensive chemical modification has little effect on the activity of avidin, making it useful for detection and protein purification. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: avID; Avidin; unnamed protein product
Gene Aliases: AVD
UniProt ID: (Chicken) P02701
Entrez Gene ID: (Chicken) 396260
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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