Immunofluorescent analysis of hnRNP-A1 in HeLa cells, fixed and permeabilized with cold methanol followed by cold methanol:acetone, using a hnRNP-A1 monoclonal antibody (Product # MA1-26736) at a dilution of 10 µg/mL followed by detection using a Goat anti-Mouse IgG, Cy3 conjugate.
|Tested species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Full length, native human hnRNPA1 protein partially purified from HeLa cells.|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
MA1-26736 detects hnRNP A1 from human samples.
MA1-26736 has been successfully used in immunoprecipitation, and Western blot procedures. This antibody does not pull down the hnRNP complex in immunoprecipitation procedures. A band of approximately 34 kDa is detected by Western Blot.
The MA1-26736 immunogen is full length, native human hnRNPA1 protein partially purified from HeLa cells.
The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variant have multiple alternative transcription initiation sites and multiple polyA sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MicroRNA-132 loss is associated with tau exon 10 inclusion in progressive supranuclear palsy.
MA1-26736 was used in western blot to study the mechanism for the tau exon mis-splicing in progressive supranuclear palsy
|Smith PY,Delay C,Girard J,Papon MA,Planel E,Sergeant N,Buée L,Hébert SS||Human molecular genetics (20:4016)||2011|
In vivo regulation of amyloid precursor protein neuronal splicing by microRNAs.
MA1-26736 was used in western blot to investigate the role of miRNAs in amyloid precursor protein splicing in vivo
|Smith P,Al Hashimi A,Girard J,Delay C,Hébert SS||Journal of neurochemistry (116:240)||2011|