|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 191 of Human hnRNP A2B1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-30960 targets hnRNP A2B1 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-30960 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 191 of Human hnRNP A2B1.
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The hnRNP-Htt axis regulates necrotic cell death induced by transcriptional repression through impaired RNA splicing.
PA5-30960 was used in western blot to study regulation of necrotic cell death induced by transcriptional repression through impaired RNA splicing by the hnRNP-Htt axis
|Mao Y,Tamura T,Yuki Y,Abe D,Tamada Y,Imoto S,Tanaka H,Homma H,Tagawa K,Miyano S,Okazawa H||Cell death and disease (7:null)||2016|