Invitrogen

hnRNP C Polyclonal Antibody

View all (13) hnRNP C antibodies

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Protocols

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hnRNP C Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of hnRNP C in HEK293 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with hnRNP C Polyclonal Antibody (Product # PA5-117991, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

hnRNP C Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human hnRNP C in human rectum with hnRNP C Polyclonal Antibody (Product # PA5-117991, 1:100 dilution, formalin-fixed paraffin embedded sections).

hnRNP C Antibody in Western Blot (WB) — Western Blot using hnRNP C Polyclonal Antibody (Product # PA5-117991) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 36 kDa.

hnRNP C Antibody in Immunoprecipitation (IP) — hnRNP C Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with hnRNP C Polyclonal Antibody (Product # PA5-117991) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: hnRNP C Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 43 kDa.

Product Details

PA5-117991

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-
Immunoprecipitation (IP)	5-10 μL/mg of lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived Human HNRNPC fragment
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography, Protein A
Storage buffer	PBS, pH 7
Contains	0.03% ProClin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2902596

Target Information

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Heterogeneous nuclear ribonucleoproteins C1/C2; hnRNP C1/C2; nuclear ribonucleoprotein particle C1 protein; nuclear ribonucleoprotein particle C2 protein

Gene Aliases: C1; C2; HNRNP; HNRNPC; HNRPC; SNRPC

UniProt ID: (Human) P07910

Entrez Gene ID: (Human) 3183

Function(s)

mRNA splicing, via spliceosome osteoblast differentiation RNA splicing gene expression protein sumoylation negative regulation of telomere maintenance via telomerase ATP-dependent chromatin remodeling 3'-UTR-mediated mRNA stabilization

Process(es)

nucleotide binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA binding mRNA 3'-UTR binding protein binding poly(U) RNA binding nucleosomal DNA binding identical protein binding poly(A) RNA binding telomerase RNA binding N6-methyladenosine-containing RNA binding

hnRNP C Polyclonal Antibody

Product Details

PA5-117991

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

References (0)

Bioinformatics

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