|Tested species reactivity||Human|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of mouse i-NOS|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Ready-to-use|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is lung tissue.
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a mesenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. Inducible NOS (iNOS) or macrophage NOS (mNOS) is a calcium/calmodulin independent and is expressed in activated macrophages and stimulated glial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Comparison of osteogenic differentiation of embryonic stem cells and primary osteoblasts revealed by responses to IL-1ß, TNF-¿, and IFN-¿.
PA1-37924 was used in immunocytochemistry to study differences in the osteogenic differentiation of murine embryonic stem cells and murine primary osteoblasts
|Sidney LE,Kirkham GR,Buttery LD||Stem cells and development (23:605)||2014|
Nicotine enhances skin necrosis and expression of inflammatory mediators in a rat pressure ulcer model.
PA1-37924 was used in western blot to study the role of nicotine in the development of pressure ulcers
|Tsutakawa S,Kobayashi D,Kusama M,Moriya T,Nakahata N||The British journal of dermatology (161:1020)||2009|