Invitrogen

iNOS Monoclonal Antibody (CXNFT), eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

37 Published Figures

42 References

View all (57) iNOS antibodies

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Datasheet

Protocols

Questions & Answers

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iNOS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Nitric oxide synthase, inducible, was performed using 70% confluent log phase RAW 264.7 cells treated LPS (1 µg/mL for 16h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with iNOS Monoclonal Antibody (CXNFT), eBioscience™ (Product # 14-5920-82) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11006), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic localization. Panel e represents untreated RAW 264.7 cells with low expression of iNOS. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.

iNOS Antibody in Western Blot (WB) — Western blot was performed using Anti-iNOS Monoclonal Antibody (CXNFT), eBioscience™ (Product # 14-5920-82) and a 130 kDa band corresponding to Nitric oxide synthase, inducible, was observed to be upregulated in RAW 264.7 cells upon treatment with LPS. Whole cell extracts (30 µg lysate) of RAW 264.7 (Lane 1) and RAW 264.7 treated with LPS (1 µg/mL for 16h) (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with F(ab2-Rabbit anti-Rat IgG (H+L Secondary Antibody, HRP (Product # PA1-29927,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

iNOS Antibody in Western Blot, Flow Cytometry (WB, Flow) — Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Western Blot and Flow Cytometry

iNOS Antibody in Flow Cytometry (Flow) — Figure 9 Flow cytometry analysis of myeloid cells in the tumors treated with SFV/IFNg, SFV/Luc, or PBS in an orthotopic 4T1 mouse breast cancer model. Mice were treated as shown in Figure 6 d. Resected tumors were homogenized to obtain a single cell suspension, which was used for immunostaining with antibodies against surface markers (CD11b, CD206, MHCII, CD38) and intracellular markers (Arginase 1, iNOs) in one mixture. ( a ) Percentages of surface markers CD11b, CD11b (high), and double-positive CD206/CD11b. ( b ) Percentages of surface markers MHCII (high) and CD11b + MHCII - . ( c ) Percentages of surface markers CD11b + /CD38 + and CD11b - /CD38 + ( d ) Percentages of intracellular markers (Arginase 1; iNOs, inducible NO synthase); representative images of Arginase 1 and iNOs gating in the CD11b + population are shown. Bars represent the means +- SD ( n = 5); * p < 0.05; ** p < 0.01; ns--nonsignificant.

iNOS Antibody — Detection of altered expression of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis using iNOS Monoclonal Antibody (CXNFT), eBioscience™ (Product # 14-5920-82), shows increased expression of iNOS upon treatment of RAW264.7 with LPS.

Product Details

14-5920-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 35 publications 35 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

CXNFT

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_2572890

Product Specific Information

Description: This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism. There are three isoforms of NOS that are encoded by three separate genes. NOS1 (neuronal NOS, nNOS) and NOS3 (endothelial NOS, eNOS) are constitutively expressed, while NOS2 is induced in response to bacterial endotoxins and inflammatory cytokines such as IFN gamma and TNF alpha. NOS2 is expressed by myeloid-derived suppressor cells and M1 macrophages but not alternatively activated M2 macrophages. NOS enzymes are functionally active only when they form homodimers, and dimerization of NOS2 occurs at steady-state concentrations of free Ca2+ such that NOS2 is functionally active when it is produced.

Applications Reported: This CXNFT antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated CXNFT is recommended for use in intracellular flow cytometry.).

Applications Tested: This CXNFT antibody has been tested by immunoblot analysis of LPS-stimulated mouse thioglycolate-elicited peritoneal exudate cells or by intracellular staining and flow cytometric analysis of mouse NOS2-transiently transfected HEK-293T cells. For immunoblot analysis this can be used at 5 µg/mL. For intracellular staining this can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nitric oxide controls proliferation of Leishmania major by inhibiting the recruitment of permissive host cells.

Immunity

Formaglio P,Alabdullah M,Siokis A,Handschuh J,Sauerland I,Fu Y,Krone A,Gintschel P,Stettin J,Heyde S,Mohr J,Philipsen L,Schröder A,Robert PA,Zhao G,Khailaie S,Dudeck A,Bertrand J,Späth GF,Kahlfuß S,Bousso P,Schraven B,Huehn J,Binder S,Meyer-Hermann M,Müller AJ

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Tue Dec 14 00:00:00 EST 2021

PD-L1 Improves Motor Function and Alleviates Neuropathic Pain in Male Mice After Spinal Cord Injury by Inhibiting MAPK Pathway.

Frontiers in immunology

Kong F,Sun K,Zhu J,Li F,Lin F,Sun X,Luo X,Ren C,Lu L,Zhao S,Sun J,Wang Y,Shi J

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

Regulation of the long noncoding RNA XIST on the inflammatory polarization of microglia in cerebral infarction.

Experimental and therapeutic medicine

Zhang M,Yang JK,Ma J

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

The thrombin receptor modulates astroglia-neuron trophic coupling and neural repair after spinal cord injury.

Glia

Kim HN,Triplet EM,Radulovic M,Bouchal S,Kleppe LS,Simon WL,Yoon H,Scarisbrick IA

14-5920-82 was used in Immunohistochemistry-immunofluorescence to demonstrate unique modulatory roles for PAR1 in regulating glial-neuron interactions, including the capacity for neurotrophic factor signaling, and underscore its position at neurobiological intersections critical for the response of the CNS to injury and the capacity for regenerative repair and restoration of function.

Wed Sep 01 00:00:00 EDT 2021

Prostaglandin E₃ attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization.

Journal of cellular and molecular medicine

Cui J,Shan K,Yang Q,Qi Y,Qu H,Li J,Wang R,Jia L,Chen W,Feng N,Chen YQ

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

STING activation normalizes the intraperitoneal vascular-immune microenvironment and suppresses peritoneal carcinomatosis of colon cancer.

Journal for immunotherapy of cancer

Lee SJ,Yang H,Kim WR,Lee YS,Lee WS,Kong SJ,Lee HJ,Kim JH,Cheon J,Kang B,Chon HJ,Kim C

14-5920-82 was used in Flow Cytometry to elucidate the immune-evasion mechanisms during the peritoneal carcinomatosis of colon cancer and developed a novel immunotherapy by activating the stimulator of interferon genes (STING) pathway.

Tue Jun 01 00:00:00 EDT 2021

Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.

Journal of neuroinflammation

Xu X,Gao W,Li L,Hao J,Yang B,Wang T,Li L,Bai X,Li F,Ren H,Zhang M,Zhang L,Wang J,Wang D,Zhang J,Jiao L

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Immunocytochemistry

Sat May 22 00:00:00 EDT 2021

TREM2 is a receptor for non-glycosylated mycolic acids of mycobacteria that limits anti-mycobacterial macrophage activation.

Nature communications

Iizasa E,Chuma Y,Uematsu T,Kubota M,Kawaguchi H,Umemura M,Toyonaga K,Kiyohara H,Yano I,Colonna M,Sugita M,Matsuzaki G,Yamasaki S,Yoshida H,Hara H

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Fri Apr 16 00:00:00 EDT 2021

Exosomes from human umbilical cord mesenchymal stem cells attenuate the inflammation of severe steroid-resistant asthma by reshaping macrophage polarization.

Stem cell research & therapy

Dong B,Wang C,Zhang J,Zhang J,Gu Y,Guo X,Zuo X,Pan H,Hsu AC,Wang G,Wang F

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Wed Mar 24 00:00:00 EDT 2021

Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection.

Immunity

Xu W,Snell LM,Guo M,Boukhaled G,Macleod BL,Li M,Tullius MV,Guidos CJ,Tsao MS,Divangahi M,Horwitz MA,Liu J,Brooks DG

14-5920-82 was used in Miscellaneous to examine how chronic viral infections alter the pulmonary microenvironment to foster coinfection and worsen disease severity.

Tue Mar 09 00:00:00 EST 2021

Mycobacterium tuberculosis-infected alveolar epithelial cells modulate dendritic cell function through the HIF-1α-NOS2 axis.

Journal of leukocyte biology

Rodrigues TS,Alvarez ARP,Gembre AF,Forni MFPAD,de Melo BMS,Alves Filho JCF,Câmara NOS,Bonato VLD

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Anticancer Activity of Liquid Treated with Microwave Plasma-Generated Gas through Macrophage Activation.

Oxidative medicine and cellular longevity

Lee CB,Seo IH,Chae MW,Park JW,Choi EH,Uhm HS,Baik KY

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Proimmunogenic impact of MEK inhibition synergizes with agonist anti-CD40 immunostimulatory antibodies in tumor therapy.

Nature communications

Baumann D,Hägele T,Mochayedi J,Drebant J,Vent C,Blobner S,Noll JH,Nickel I,Schumacher C,Boos SL,Daniel AS,Wendler S,Volkmar M,Strobel O,Offringa R

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

CCL2 and CXCL12 Derived from Mesenchymal Stromal Cells Cooperatively Polarize IL-10+ Tissue Macrophages to Mitigate Gut Injury.

Cell reports

Giri J,Das R,Nylen E,Chinnadurai R,Galipeau J

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Tue Feb 11 00:00:00 EST 2020

Modulation of the functions of myeloid-derived suppressor cells : a new strategy of hydrogen sulfide anti-cancer effects.

British journal of pharmacology

De Cicco P,Ercolano G,Rubino V,Terrazzano G,Ruggiero G,Cirino G,Ianaro A

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells.

Journal of cellular and molecular medicine

Wu R,Liu C,Deng X,Chen L,Hao S,Ma L

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Transmembrane Tumor Necrosis Factor Controls Myeloid-Derived Suppressor Cell Activity via TNF Receptor 2 and Protects from Excessive Inflammation during BCG-Induced Pleurisy.

Frontiers in immunology

Chavez-Galan L,Vesin D,Uysal H,Blaser G,Benkhoucha M,Ryffel B,Quesniaux VFJ,Garcia I

14-5920 was used in Flow cytometry/Cell sorting to provide insight into the crucial role played by the transmembrane TNF/TNF receptor 2 pathway in myeloid-derived suppressor cell suppressive activity required during acute pleural infection to attenuate excessive inflammation generated by the infection.

Wed Nov 20 00:00:00 EST 2019

AMPK Alpha-1 Intrinsically Regulates the Function and Differentiation of Tumor Myeloid-Derived Suppressor Cells.

Cancer research

Trillo-Tinoco J,Sierra RA,Mohamed E,Cao Y,de Mingo-Pulido Á,Gilvary DL,Anadon CM,Costich TL,Wei S,Flores ER,Ruffell B,Conejo-Garcia JR,Rodriguez PC

14-5920-82 was used in Flow cytometry/Cell sorting to elucidate the intrinsic interaction between the expression of AMP-activated protein kinase alpha (AMPKα) and the immunoregulatory activity of myeloid-derived suppressor cells in tumors.

Tue Oct 01 00:00:00 EDT 2019

1,25‑Dihydroxy‑Vitamin D3 induces macrophage polarization to M2 by upregulating T‑cell Ig‑mucin‑3 expression.

Molecular medicine reports

Liang S,Cai J,Li Y,Yang R

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Western Blot

Wed May 01 00:00:00 EDT 2019

1,25‑Dihydroxy‑Vitamin D3 induces macrophage polarization to M2 by upregulating T‑cell Ig‑mucin‑3 expression.

Molecular medicine reports

Liang S,Cai J,Li Y,Yang R

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Western Blot

Wed May 01 00:00:00 EDT 2019

Innate immune memory through TLR2 and NOD2 contributes to the control of Leptospira interrogans infection.

PLoS pathogens

Santecchia I,Vernel-Pauillac F,Rasid O,Quintin J,Gomes-Solecki M,Boneca IG,Werts C

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Adoptive transfer of Trichinella spiralis-activated macrophages can ameliorate both Th1- and Th2-activated inflammation in murine models.

Scientific reports

Kang SA,Park MK,Park SK,Choi JH,Lee DI,Song SM,Yu HS

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Thu Apr 25 00:00:00 EDT 2019

Preventive Effects of Different Fermentation Times of Shuidouchi on Diphenoxylate-Induced Constipation in Mice.

Foods (Basel, Switzerland)

Chen L,Zhang J,Suo H,Wang W,Wang H,Zhang Y,Hu Q,Zhao X,Li J

14-5920-82 was used in Western Blot to compare the prevention effects of Shuidouchi with different fermentation times on constipation in mice.

Fri Mar 01 00:00:00 EST 2019

Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses.

Nature communications

Kurelac I,Iommarini L,Vatrinet R,Amato LB,De Luise M,Leone G,Girolimetti G,Umesh Ganesh N,Bridgeman VL,Ombrato L,Columbaro M,Ragazzi M,Gibellini L,Sollazzo M,Feichtinger RG,Vidali S,Baldassarre M,Foriel S,Vidone M,Cossarizza A,Grifoni D,Kofler B,Malanchi I,Porcelli AM,Gasparre G

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Fri Feb 22 00:00:00 EST 2019

Oncostatin M is overexpressed in skin squamous-cell carcinoma and promotes tumor progression.

Oncotarget

Simonneau M,Frouin E,Huguier V,Jermidi C,Jégou JF,Godet J,Barra A,Paris I,Levillain P,Cordier-Dirikoc S,Pedretti N,Bernard FX,Lecron JC,Morel F,Favot L

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Fri Nov 23 00:00:00 EST 2018

Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge.

Nature communications

Yi S,Zhai J,Niu R,Zhu G,Wang M,Liu J,Huang H,Wang Y,Jing X,Kang L,Song W,Shi Y,Tang H

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Mon Sep 24 00:00:00 EDT 2018

Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype.

Nature communications

Chen D,Xie J,Fiskesund R,Dong W,Liang X,Lv J,Jin X,Liu J,Mo S,Zhang T,Cheng F,Zhou Y,Zhang H,Tang K,Ma J,Liu Y,Huang B

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Western Blot

Wed Feb 28 00:00:00 EST 2018

Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype.

Nature communications

Chen D,Xie J,Fiskesund R,Dong W,Liang X,Lv J,Jin X,Liu J,Mo S,Zhang T,Cheng F,Zhou Y,Zhang H,Tang K,Ma J,Liu Y,Huang B

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Western Blot

Wed Feb 28 00:00:00 EST 2018

Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency.

Nature cell biology

Giampazolias E,Zunino B,Dhayade S,Bock F,Cloix C,Cao K,Roca A,Lopez J,Ichim G,Proïcs E,Rubio-Patiño C,Fort L,Yatim N,Woodham E,Orozco S,Taraborrelli L,Peltzer N,Lecis D,Machesky L,Walczak H,Albert ML,Milling S,Oberst A,Ricci JE,Ryan KM,Blyth K,Tait SWG

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Phenotypic switch in lung interstitial macrophage polarization in an ovalbumin-induced mouse model of asthma.

Experimental and therapeutic medicine

Nie H,Wang A,He Q,Yang Q,Liu L,Zhang G,Huang Y,Ding X,Yu H,Hu S

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Phenotypic switch in lung interstitial macrophage polarization in an ovalbumin-induced mouse model of asthma.

Experimental and therapeutic medicine

Nie H,Wang A,He Q,Yang Q,Liu L,Zhang G,Huang Y,Ding X,Yu H,Hu S

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Phenotypic switch in lung interstitial macrophage polarization in an ovalbumin-induced mouse model of asthma.

Experimental and therapeutic medicine

Nie H,Wang A,He Q,Yang Q,Liu L,Zhang G,Huang Y,Ding X,Yu H,Hu S

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

IL-10 Plays Opposing Roles during Staphylococcus aureus Systemic and Localized Infections.

Journal of immunology (Baltimore, Md. : 1950)

Leech JM,Lacey KA,Mulcahy ME,Medina E,McLoughlin RM

14592082 was used in flow cytometry to define the functions of IL-10 during Staphylococcus aureus

Wed Mar 15 00:00:00 EDT 2017

Liver Necrosis and Lethal Systemic Inflammation in a Murine Model of Rickettsia typhi Infection: Role of Neutrophils, Macrophages and NK Cells.

PLoS neglected tropical diseases

Papp S,Moderzynski K,Rauch J,Heine L,Kuehl S,Richardt U,Mueller H,Fleischer B,Osterloh A

14592082 was used in flow cytometry to investigate the contribution of neutrophils and macrophages during Rickettsia typhi infection

Mon Aug 01 00:00:00 EDT 2016

OGR1/GPR68 Modulates the Severity of Experimental Autoimmune Encephalomyelitis and Regulates Nitric Oxide Production by Macrophages.

PloS one

D'Souza CA,Zhao FL,Li X,Xu Y,Dunn SE,Zhang L

14-5920 was used in Flow cytometry/Cell sorting to suggest that ovarian cancer G protein-coupled receptor 1 plays a key role in regulating T cell responses during autoimmunity.

Tue Jul 19 00:00:00 EDT 2016

TGF-β receptor 1 inhibition prevents stenosis of tissue-engineered vascular grafts by reducing host mononuclear phagocyte activation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Lee YU,de Dios Ruiz-Rosado J,Mahler N,Best CA,Tara S,Yi T,Shoji T,Sugiura T,Lee AY,Robledo-Avila F,Hibino N,Pober JS,Shinoka T,Partida-Sanchez S,Breuer CK

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

TNF-Mediated Restriction of Arginase 1 Expression in Myeloid Cells Triggers Type 2 NO Synthase Activity at the Site of Infection.

Cell reports

Schleicher U,Paduch K,Debus A,Obermeyer S,König T,Kling JC,Ribechini E,Dudziak D,Mougiakakos D,Murray PJ,Ostuni R,Körner H,Bogdan C

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Tue May 03 00:00:00 EDT 2016

Persisting Rickettsia typhi Causes Fatal Central Nervous System Inflammation.

Infection and immunity

Osterloh A,Papp S,Moderzynski K,Kuehl S,Richardt U,Fleischer B

14592082 was used in flow cytometry to report the course of Rickettsia typhi infection in RAG1 knockout mice

Sun May 01 00:00:00 EDT 2016

Loss of SOCS3 in myeloid cells prolongs survival in a syngeneic model of glioma.

Oncotarget

McFarland BC,Marks MP,Rowse AL,Fehling SC,Gerigk M,Qin H,Benveniste EN

14592082 was used in flow cytometry to observe that Suppressor of Cytokine Signaling 3-deficient bone marrow-derived macrophages display enhanced and prolonged expression of pro-inflammatory M1 cytokines when exposed to glioma tumor cell conditioned medium in vitro

Tue Apr 12 00:00:00 EDT 2016

Maternal immune activation evoked by polyinosinic:polycytidylic acid does not evoke microglial cell activation in the embryo.

Frontiers in cellular neuroscience

Smolders S,Smolders SM,Swinnen N,Gärtner A,Rigo JM,Legendre P,Brône B

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Mon Aug 24 00:00:00 EDT 2015

Tasquinimod modulates suppressive myeloid cells and enhances cancer immunotherapies in murine models.

Cancer immunology research

Shen L,Sundstedt A,Ciesielski M,Miles KM,Celander M,Adelaiye R,Orillion A,Ciamporcero E,Ramakrishnan S,Ellis L,Fenstermaker R,Abrams SI,Eriksson H,Leanderson T,Olsson A,Pili R

Published figure using iNOS monoclonal antibody (Product # 14-5920-82) in Flow Cytometry

Sun Feb 01 00:00:00 EST 2015

Commensal Gram-positive bacteria initiates colitis by inducing monocyte/macrophage mobilization.

Mucosal immunology

Nakanishi Y,Sato T,Ohteki T

Thu Jan 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: hepatocytes; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible, macrophage; Nitric oxide synthase, inducible; nitric oxide synthase-inducible; NOS type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2

Gene Aliases: i-NOS; iNOS; Inosl; Nos-2; NOS-II; Nos2; Nos2a

UniProt ID: (Mouse) P29477

Entrez Gene ID: (Mouse) 18126

Molecular Function: oxidoreductase

Function(s)

ovulation from ovarian follicle response to hypoxia arginine catabolic process superoxide metabolic process nitric oxide biosynthetic process inflammatory response nitric oxide mediated signal transduction negative regulation of gene expression peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity response to lipopolysaccharide cellular response to drug regulation of cell proliferation negative regulation of protein catabolic process defense response to bacterium negative regulation of blood pressure regulation of protein kinase activity positive regulation of vasodilation regulation of insulin secretion positive regulation of killing of cells of other organism oxidation-reduction process cellular response to lipopolysaccharide cellular response to interferon-gamma

Process(es)

actin binding NADPH-hemoprotein reductase activity nitric-oxide synthase activity receptor binding iron ion binding protein binding calmodulin binding beta-catenin binding cAMP-dependent protein kinase regulator activity FMN binding oxidoreductase activity protein kinase binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity cadherin binding metal ion binding flavin adenine dinucleotide binding nitric-oxide synthase binding Hsp90 protein binding

iNOS Monoclonal Antibody (CXNFT), eBioscience™

Product Details

14-5920-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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