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Immunofluorescent analysis of insulin degrading enzyme showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using an Insulin degrading enzyme polyclonal antibody (Product # PA5-29350) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 349 and 635 of Human insulin degrading enzyme|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29350 targets insulin degrading enzyme in IHC (P), ICC and WB applications and shows reactivity with Human, mouse and rat samples.
The PA5-29350 immunogen is recombinant fragment corresponding to a region within amino acids 349 and 635 of Human insulin degrading enzyme.
This gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Abeta-degrading protease; insulin protease; insulin-degrading enzyme; insulinase; INSULYSIN
1300012G03Rik; 4833415K22Rik; AA675336; AI507533; IDE; INSDEGM; INSULYSIN