Immunofluorescent analysis of insulin degrading enzyme showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using an Insulin degrading enzyme polyclonal antibody (Product # PA5-29350) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 349 and 635 of Human insulin degrading enzyme|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29350 targets insulin degrading enzyme in IHC (P), ICC and WB applications and shows reactivity with Human, mouse and rat samples.
The PA5-29350 immunogen is recombinant fragment corresponding to a region within amino acids 349 and 635 of Human insulin degrading enzyme.
This gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.