|Tested species reactivity||Bovine, Dog, Horse, Human, Mouse, Non-human primate, Pig, Rat, Xenopus|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues 96-113 of human kB-Ras1.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: 293 whole cell lysate.
NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first genes induced following NF-kB activation is IkBa. Recently, two proteins, kB-Ras1 and kB-Ras2 have been identified (7). These two proteins interact with PEST domains of IkBa and IkBb and decrease their rate of degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.