Immunofluorescent analysis of karyopherin alpha 2 showing staining in the cytoplasm and nucleus of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a karyopherin alpha 2 polyclonal antibody (Product # PA5-21748) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 468 and 529 of KPNA2 (Uniprot ID#P52292)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21748 targets karyopherin alpha 2 in IF and WB applications and shows reactivity with Human samples.
The PA5-21748 immunogen is synthetic peptide contain a sequence corresponding to a region within amino acids 468 and 529 (P52292) of KPNA2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.