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Immunofluorescent analysis of lactate dehydrogenase C showing staining in the cytoplasm of NT2D1 cells. NT2D1 cells were fixed in ice-cold MeOH for 5 min and stained using a lactate dehydrogenase C polyclonal antibody (Product # PA5-30079) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 5 and 229 of Human lactate dehydrogenase C|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30079 targets lactate dehydrogenase C in WB applications and shows reactivity with Human samples.
The PA5-30079 immunogen is recombinant fragment corresponding to a region within amino acids 5 and 229 of Human lactate dehydrogenase C.
Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cancer/testis antigen 32; CT32; L-lactate dehydrogenase C chain; lactate dehydrogenase c variant 1; lactate dehydrogenase c variant 3; lactate dehydrogenase c variant 4; lactate dehydrogenase c variant 5; lactate dehydrogenase C4; LDH testis subunit; LDH-C; LDH-X; LDH3; LDHX; testis secretory sperm-binding protein Li 234P
CT32; LDH3; LDHC; LDHX