Immunofluorescent analysis of lactate dehydrogenase C showing staining in the cytoplasm of NT2D1 cells. NT2D1 cells were fixed in ice-cold MeOH for 5 min and stained using a lactate dehydrogenase C polyclonal antibody (Product # PA5-30079) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 5 and 229 of Human lactate dehydrogenase C|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30079 targets lactate dehydrogenase C in WB applications and shows reactivity with Human samples.
The PA5-30079 immunogen is recombinant fragment corresponding to a region within amino acids 5 and 229 of Human lactate dehydrogenase C.
Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region.
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