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Intestinal tissue from a transgenic mouse expressing mCherry in all tissues was isolated and fixed in 4% paraformaldehyde. mCherry Rat Monoclonal Antibody (M11217) was used at a 1:15,000 dilution. Using the ImmPRESS™ Anti-Rat Ig (peroxidase) Polymer Detection Kit (Vector Laboratories) and following the manufacturer"e;s instructions, the sections were incubated in peroxidase substrate solution until the desired stain intensity developed. A) Fluorescent image detecting mCherry expression and B) HRP-stained image.
|Tested species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||full-length protein mCherry|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Because of its improved brightness, superior photostability, and extremely rapid maturation rate, the mCherry monomeric red fluorescent protein is becoming the red fluorescent protein of choice for monitoring physiological processes and detecting transgenic expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Molecular evolution and in vitro characterization of Botryllus histocompatibility factor.
M11217 was used in immunocytochemistry and western blot to study allorecognition using Botryllus schlosseri.
|Taketa DA,Nydam ML,Langenbacher AD,Rodriguez D,Sanders E,De Tomaso AW||Immunogenetics (67:605)||2015|
|Not Applicable||Not Cited||
ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function.
M11217 was used in immunoprecipitation to uncover a role for ATG12-ATG3 in late endosome function that is distinct from autophagy.
|Murrow L,Malhotra R,Debnath J||Nature cell biology (17:300)||2015|