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U2OS cells were transduced using an adenoviral construct expressing mCherry.<br> A) Native expression of mCherry detected post-transduction using Texas Red filters (562 nm/624 nm)<br> B) Anti-Cherry antibody added and cells imaged using the Cy5 filter set (628 nm/692 nm)<br> C) mCherry expression detected by adding anti-mCherry and Alexa Fluor® 647 goat anti-rat (A21247)
|Tested species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||full-length protein mCherry|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Because of its improved brightness, superior photostability, and extremely rapid maturation rate, the mCherry monomeric red fluorescent protein is becoming the red fluorescent protein of choice for monitoring physiological processes and detecting transgenic expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Molecular evolution and in vitro characterization of Botryllus histocompatibility factor.
M11217 was used in immunocytochemistry and western blot to study allorecognition using Botryllus schlosseri.
|Taketa DA,Nydam ML,Langenbacher AD,Rodriguez D,Sanders E,De Tomaso AW||Immunogenetics (67:605)||2015|