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mGLuR4 Antibody (702603) in ICC/IF
For immunofluorescence analysis, HEK-293 cells were stably transfected with human mGLuR4. After 24hr of transfection the cells were fixed and permeabilized for detection of endogenous mGLuR4 using Anti-mGLuR4 Recombinant Rabbit Monoclonal Antibody (Product # 702603, 1 µg/mL, 1:500 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate. Panel a) shows representative cells that were stained for detection of membrane localization of the mGLuR4 on the transfected cells. The images were... View More
Product Details
702603
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
6H26L4
Immunogen
Peptide corresponding to human mGLUR4 [aa891-aa912]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2734819
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Rabbit, Cat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The Metabotropic Glutamate Receptor mGluR4/GRM4 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glutamate receptor; glutamate receptor, metabotropic 4; Metabotropic glutamate receptor 4; mGluR4
Gene Aliases: GPRC1D; GRM4; mGlu4; MGLUR4
UniProt ID: (Human) Q14833, (Rat) P31423
Entrez Gene ID: (Human) 2914, (Rat) 24417
activation of MAPK activity
adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway
chemical synaptic transmission
neurotransmitter secretion
positive regulation of MAPK cascade
regulation of neuron apoptotic process
regulation of synaptic transmission, glutamatergic
learning
negative regulation of cell proliferation
gamma-aminobutyric acid secretion
neuron projection development
synaptic transmission, glutamatergic
negative regulation of apoptotic process
negative regulation of synaptic transmission
synaptic transmission, GABAergic
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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