|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues: S(1159) S S VP S S P V S E S V L(1171) of the human GluR1 protein.|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-500|
|Western Blot (WB)||1:500-1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-4516 detects Metabotropic Glutamate Receptor 1 in human, rat, and mouse samples.
PA1-4516 has been successfully used in Western blot and Immunohistochemistry procedures.
PA1-4516 immunogen is a synthetic peptide corresponding to residues 1159-1171 of the human GluR1 protein.
Maintain at 4°C for 3 months or at -20°C for longer periods. Avoid freeze/thaw cycles.
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. The Glutamate Receptor, Metabotropic, 1, (mGluR1 alpha) activates phospholipase C and participates in the central action of glutamate in the central nervous system, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Metabotropic glutamate receptor 1 (mGluR1): antibody specificity and receptor expression in cultured primary neurons.
PA1-4516 was used in western blot to study the expression of metabotropic glutamate receptor 1
|Ayala R,Kett LR,Leach TL,Young AB,Dunah AW,Orlando LR||Journal of neuroscience methods (204:221)||2012|