|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (commercially sensitive amino acid sequence) (Human) (C-terminal).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-21039 detects Metabotropic Glutamate Receptor 6 and 7 in Human, Mouse, Rat and Non-human primate samples.
PA1-21039 has been successfully used in Immunohistochemistry (Paraffin), Immunoprecipitation and Western blot procedures. For immunohistochemistry (paraffin), staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 minutes.
PA1-21039 immunogen corresponds to synthetic peptide (commercially sensitive amino acid sequence) (Human) (C-terminal).
Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: Ionotropic receptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR17, NMDA13, KAI2), which comprise ligand-gated calcium channels. Metabotropic glutamate receptors are G-protein coupled receptors, which when activated can activate phopholipases or adenylylcyclase, depending on the neuron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.