Display Info:
cookie.CK_ISO_CODE.value:us
cookie.CK_LANG_CODE.value:en
cookie.CK_DISPLAY_TYPE.value:
pageContext.request:org.apache.struts2.dispatcher.StrutsRequestWrapper@2576b1e8
Request attributes: pageContext.request.scheme:http(not used)
syndicationProtocol:https
syndicationExtensionPrefix:.secure
syndicationDisplayType:
      LOADING ...

      System Message

      OKCancel

      mTOR Monoclonal Antibody (215Q18)

      See 18 other mTOR antibodies ›
      mTOR Monoclonal Antibody for Western Blot, IF, ICC and IHC (P)

      Catalog#: AHO1232 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      AHO1232 100 µg
      304.00
      Please enter a quantity in the range of 1 to 199.
      Suggested Secondary Antibodies

      Anti-Mouse Secondary Antibodies (597)
      Alexa Fluor® Anti-Mouse Secondary Antibodies (122)
      HRP Anti-Mouse Secondary Antibodies (86)

      Product Details

      References

      Bioinformatics

      Documents
      Details
      Tested species reactivity Human, Mouse, Rat
      Published species reactivity Rat
      Host / Isotype Mouse / IgG2b, kappa
      Class Monoclonal
      Type Antibody
      Clone 215Q18
      Immunogen Recombinant fragment of human mTOR expressed in E. coli.
      Conjugate Unconjugated
      Form Liquid
      Purification Affinity chromatography
      Storage buffer PBS, pH 7.2, with 1% BSA
      Contains 0.1% sodium azide
      Storage Conditions -20°C
      Tested Applications Dilution *
      Immunocytochemistry (ICC) 1:100-1:500
      Immunofluorescence (IF) 1:100-1:500
      Immunohistochemistry (Paraffin) (IHC (P)) 1:10-1:100
      Western Blot (WB) Assay Dependent

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Published Applications
      Western Blot (WB) See 1 publications below

      Background/Target Information

      Mammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3-kinase and Akt/PKB signaling, resulting in phosphorylation of 4EBP1, and initiation of mRNA translation. A second pathway involves regulation of ribosomal S6 kinase, which affects ribosome biogenesis and translation elongation.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References

      Western Blot

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Rat 1:500 Rapamycin reveals an mTOR-independent repression of Kv1.1 expression during epileptogenesis.
      AHO1232 was used in western blot to study mTOR, microRNA miR-129-5p, and Kv1.1 in epilepsy       		Sosanya NM,Brager DH,Wolfe S,Niere F,Raab-Graham KF Neurobiology of disease (73:96) 2015

      Bioinformatics

      Protein Aliases:

      angiopoietin-like factor CDT6; EC 2.7.11.1; FK506 binding protein 12-rapamycin associated protein; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-Rapamycin Associated Protein; FKBP-rapamycin associated protein (FRAP); FKBP-rapamycin-associated protein FRAP; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; FRAP1; FRAP2; m-TOR; Mammalian Target Of Rapamycin; Mechanistic Target Of Rapamycin; mechanistic target of rapamycin (serine/threonine kinase); MTOR; mTORC1; RAFT1; Rapamycin and FKBP12 target 1; rapamycin and FKBP12 target-1 protein; rapamycin associated protein FRAP2; Rapamycin Target Protein; Rapamycin target protein 1; RAPT1; Serine/Threonine-Protein Kinase MTOR

      Gene Aliases:

      2610315D21Rik; AI327068; flat; FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS

      UniProt ID:

      (Human) P42345, (Mouse) Q9JLN9, (Rat) P42346

      Entrez Gene ID:

      (Human) 2475, (Mouse) 56717, (Rat) 56718

      Molecular Function:

      kinase non-receptor serine/threonine protein kinase nucleic acid binding nucleotide kinase protein kinase transferase

      Function(s) Process(es)

      Documents

      Material safety data sheets (MSDS)

      Display Info:
      cookie.CK_ISO_CODE.value:us
      cookie.CK_LANG_CODE.value:en
      cookie.CK_DISPLAY_TYPE.value:
      pageContext.request.scheme:http(not used)
      syndicationProtocol:https
      syndicationExtensionPrefix:.secure
      syndicationDisplayType: