|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a C-terminal region (within residues 250-294) of human mutant nucleophosmin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.0|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Suggested positive control: antigen standard for NPM1 (transient overexpression lysate), OCI-AML3 whole cell lysate.
HL-60 lysate can be used as a negative control for wildtype protein.
Nucleophosmin (NPM; also known as B23, numatrin or NO38) is a highly conserved phosphoprotein that belongs to nucleoplasmin/nucleophosmin family of nuclear chaperones. Expressed ubiquitously with nucleolar localization, NPM shuttles between nucleoli and cytoplasm during cell cycle progression. It binds ribosome to drive ribosome nuclear export and has been associated with nucleolar ribonucleoprotein structures. Chaperonin are the proteins that provide favourable conditions for the correct folding of other proteins for preventing aggregation; and NPM has the ability to acts as chaperonin for the core histones H3, H2B and H4 proteins. NPM stimulates APEX1 endonuclease activity on A/P double-stranded DNA while inhibit the same on A/P single-stranded RNA, and regulate APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA as well as the removal of oxidized rRNA molecules. BRCA2 helps NPM in regulating centrosome duplication, whereas, PLK2 mediated phosphorylation controls the centriole duplication. NPM is implicated in diverse cellular processes including cell proliferation, and regulation of tumor suppressors such as p53/TP53 and ARF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Role of cysteine 288 in nucleophosmin cytoplasmic mutations: sensitization to toxicity induced by arsenic trioxide and bortezomib.
PA1-46356 was used in immunocytochemistry to study the increased sensitivity to arsenic trioxide and bortezomib of cells bearing the nucleophosmin cysteine 288 mutation
|Huang M,Thomas D,Li MX,Feng W,Chan SM,Majeti R,Mitchell BS||Leukemia (27:1970)||2013|