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|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Synthetic peptide conjugated to KLH, corresponding to amino acids 327-339 of human N-myc.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:10-1:500|
|Gel Shift (GS)||1:1-1:100|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for MYCN (transient overexpression lysate).
The v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins of 64 kDa and 67 kDa that exhibit relatively short (30 minute) half lives in vivo and exhibit DNA binding properties in vitro. Members of the Myc gene family code for proteins that homodimerize but do not bind well to DNA. Myc-Max heterodimers, however, bind with great affinity to the sequence CACGTG and act to initiate transcription of target genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
class E basic helix-loop-helix protein 37; MYCN; N-myc proto-oncogene protein; neuroblastoma MYC oncogene; neuroblastoma myc-related oncogene 1; neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene; NMYC proto oncogene protein; oncogene NMYC; pp65/67
BHLHE37; c-nmyc; MODED; MYCN; N-myc; NMYC; Nmyc-1; Nmyc1; ODED