Western blot analysis of nm23-H2 using A) 30 µg A549 whole cell extract (B) 30 µg H1299 whole cell extract and C) 30 µg HCT116 whole cell extract. Samples were loaded onto a 12% SDS-PAGE gel and probed with a nm23-H2 polyclonal antibody (Product # PA5-27488) at a dilution of 1:1000.
|Tested species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 105 of Human nm23-H2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 40% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27488 targets nm23-H2 in Western blot applications and shows reactivity with Human and mouse samples.
The PA5-27488 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 105 of Human nm23-H2.
Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product.
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