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Immunohistochemistry was performed on cancer biopsies of deparaffinized human Bladder carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing nmt55 / p54nrb (Product #MA3-2024) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
|Tested species reactivity||Human, Rabbit, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues T(140) V R E A G P P A F Y R P N S(154) of human ER alpha.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-2024 detects the nmt55/p54nrb in human, rat and rabbit samples.
MA3-2024 has been successfully used in immunohistochemistry, immunoprecipitation, and Western blot procedures. By Western blot, this antibody detects a ~55 kDa protein representing nmt55/p54nrb in MCF-7 cells, human breast tumor cells, rat uterine tissue, and rabbit liver extracts. In immunohistochemistry this antibody recognizes the nmt55/p54nrb in MCF-7 cells and human breast tumor cells. This monoclonal antibody has been referred to as
The MA3-2024 immunogen is a synthetic peptide corresponding to residues T(140) V R E A G P P A F Y R P N S(154) of human ER alpha.
Nmt55/p54nrb is an RNA binding protein that has been implicated in multiple cellular functions such as regulation of cell division as well as cancer biology. Expression of this protein has been found to be decreased in some human breast tumors, and has been studied as a relevant tumor marker. It has been found, that decreased nmt55/p54nrb expression in Estrogen Receptor (ER) negative human breast tumors or the expression of nmt55/p54nrb variants in ER positive tumors may indicate the loss of normal growth. It is possible that nmt55/p54nrb acts as a pre-mRNA splicing factor affecting the structure and function of hERalpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
TRAP150 interacts with the RNA-binding domain of PSF and antagonizes splicing of numerous PSF-target genes in T cells.
MA3-2024 was used in western blot to investigate the interaction between TRAP150 and PSF and its effect in T cells
|Yarosh CA,Tapescu I,Thompson MG,Qiu J,Mallory MJ,Fu XD,Lynch KW||Nucleic acids research (43:9006)||2015|
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
MA3-2024 was used in western blot to investigate the mechanism for signal-regulated alternative splicing
|Melton AA,Jackson J,Wang J,Lynch KW||Molecular and cellular biology (27:6972)||2007|
p54nrb is a component of the snRNP-free U1A (SF-A) complex that promotes pre-mRNA cleavage during polyadenylation.
MA3-2024 was used in western blot to investigate the roles of the p54nrb during the pre-mRNA cleavage.
|Liang S,Lutz CS||RNA (New York, N.Y.) (12:111)||2006|
Multisite phosphorylation of Pin1-associated mitotic phosphoproteins revealed by monoclonal antibodies MPM-2 and CC-3.
MA3-2024 was used in western blot to investigate the phosphorylation pattern of mitotic phosphoprotein
|Albert AL,Lavoie SB,Vincent M||BMC cell biology (5:null)||2004|
Immunodetection of nmt55/p54nrb isoforms in human breast cancer.
MA3-2024 was used in immunohistochemistry to investigate the expression level of nmt55/p54nrb isoforms in human breast cancer
|Pavao M,Huang YH,Hafer LJ,Moreland RB,Traish AM||BMC cancer (1:null)||2002|
54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex, 52 kDa subunit; NMT-1; NMT55; non-POU domain containing, octamer-binding-like; non-POU domain-containing octamer (ATGCAAAT) binding protein; Non-POU domain-containing octamer-binding protein; non-POU-domain-containing, octamer-binding protein; NonO protein; p54(nrb); p54nrb; protein phosphatase 1, regulatory subunit 114
MRXS34; NMT55; NONO; NRB54; P54; P54NRB; PPP1R114