|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Activated peripheral blood nuclei.|
|Storage buffer||PBS, pH 7.2, with 0.02% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:100-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-26934 detects p105 from human samples.
MA1-26934 has been successfully used in immunohistochemistry (paraffin and frozen sections), and Western blot procedures. Formalin-fixed paraffin-embedded sections require pepsin or trypsin digestion prior to staining.
The MA1-26934 immunogen is activated peripheral blood nuclei.
The molecular weight of p105 is 105 kDa for the dimeric form and 41 kDa for the monomeric form.
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.