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Immunofluorescence analysis of p120 Catenin (C-Term) was done on 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with p120 Catenin (C-Term) Mouse Monoclonal Antibody (339600) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic and nuclear localization of p120 Catenin (C-Term). Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant fusion protein derived from the C-terminus of murine p120ct|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and althernative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Pro-Tumorigenic Phosphorylation of p120 Catenin in Renal and Breast Cancer.
33-9600 was used in western blot to study the role of p20 phosphorylation in tumor transformation.
|Kourtidis A,Yanagisawa M,Huveldt D,Copland JA,Anastasiadis PZ||PloS one (10:null)||2015|
|Mouse||Not Cited||Conditional targeting of E-cadherin in skin: insights into hyperproliferative and degenerative responses.||Tinkle CL,Lechler T,Pasolli HA,Fuchs E||Proceedings of the National Academy of Sciences of the United States of America (101:552)||2004|
|Human||Not Cited||Regulation of cell-cell interactions by phosphatidic acid phosphatase 2b/VCIP.||Humtsoe JO,Feng S,Thakker GD,Yang J,Hong J,Wary KK||The EMBO journal (22:1539)||2003|
||Conditional targeting of E-cadherin in skin: insights into hyperproliferative and degenerative responses.||Tinkle CL,Lechler T,Pasolli HA,Fuchs E||Proceedings of the National Academy of Sciences of the United States of America (101:552)||2004|
cadherin-associated Src substrate; CAS; catenin (cadherin-associated protein), delta 1; catenin delta-1; catenin src; CTNND; p120 catenin; p120(cas); p120(ctn); p120-catenin; P120CAS; P120CTN
AA409437; AU019353; CAS; Catns; CTNND; CTNND1; KIAA0384; mKIAA0384; P120; p120(CAS); p120(CTN); P120CAS; P120CTN