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Immunofluorescence analysis of p120 Catenin (N-Term) was done on 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with p120 Catenin (N-Term) Mouse Monoclonal Antibody (339700) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing junctional localization of p120 Catenin (N-Term) and panel e is a no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Dog, Chicken, Human, Mouse, Rat|
|Published species reactivity||Dog, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant fusion protein derivedfrom the N-terminus of murine p120ctn|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and althernative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cell surface heparan sulfate proteoglycans control adhesion and invasion of breast carcinoma cells.
33-9700 was used in immunocytochemistry to study the roll of cell surface heparan sulfate proteoglycans in relation to cell adhesion.
|Lim HC,Multhaupt HA,Couchman JR||Molecular cancer (14:null)||2015|
|The protein tyrosine phosphatase Pez is a major phosphatase of adherens junctions and dephosphorylates beta-catenin.||Wadham C,Gamble JR,Vadas MA,Khew-Goodall Y||Molecular biology of the cell (14:2520)||2003|
cadherin-associated Src substrate; CAS; catenin (cadherin-associated protein), delta 1; catenin delta-1; catenin src; CTNND; p120 catenin; p120(cas); p120(ctn); p120-catenin; P120CAS; P120CTN
AA409437; AU019353; CAS; Catns; CTNND; CTNND1; KIAA0384; mKIAA0384; P120; p120(CAS); p120(CTN); P120CAS; P120CTN