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|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A synthetic peptide from p130 corresponding to aa878-913|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
MA5-14305 targets p130/Rb2 in IF, IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.
The MA5-14305 immunogen is a synthetic peptide from p130 corresponding to aa878-913.
Two retinoblastoma-related proteins, p107 and pRb2/p130 are structurally and functionally similar to the product of the retinoblastoma gene (pRb/p105). Like pRb they bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain". Both proteins play a fundamental role in growth control. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2/p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Potent anti-tumor effect generated by a novel human papillomavirus (HPV) antagonist peptide reactivating the pRb/E2F pathway.
MA5-14305 was used in western blot to evaluate the therapeutic effectiveness of a human papillomavirus antagonist peptide for cancer treatment
|Guo CP,Liu KW,Luo HB,Chen HB,Zheng Y,Sun SN,Zhang Q,Huang L||PloS one (6:null)||2011|
Analysis of promoter binding by the E2F and pRB families in vivo: distinct E2F proteins mediate activation and repression.
MA5-14305 was used in western blot to study promoter binding by the E2F and pRB families in vivo
|Takahashi Y,Rayman JB,Dynlacht BD||Genes & development (14:804)||2000|
130 kDa retinoblastoma-associated protein; P130; PPAR-alpha-interacting complex protein 128; PRB2; PRIC128; RBR-2; retinoblastoma-like 2; retinoblastoma-like protein 2; retinoblastoma-related protein 2
P130; RB2; RBL2