|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human p130Cas protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14276 targets p130Cas in IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.
The MA5-14276 immunogen is recombinant human p130Cas protein.
The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins. p130Cas has a unique structure that contains a Src homology (SH)-3 domain followed by multiple YXXP motifs and a proline-rich region p130Cas is implicated in a variety of biological processes including cell adhesion, cell migration, growth factor stimulation, cytokine receptor engagement and bacterial infection. Physiological functions of p130Cas include cardiovascular development, actin filament assembly and Src-induced cell transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ezrin and BCAR1/p130Cas mediate breast cancer growth as 3-D spheroids.
MA5-14276 was used in immunohistochemistry to study the role of ezrin and BCAR1/p130Cas in mediating breast cancer growth as 3-D spheroids and in breast carcinoma progression
|Konstantinovsky S,Davidson B,Reich R||Clinical and experimental metastasis (29:527)||2012|
Breast carcinoma cells in primary tumors and effusions have different gene array profiles.
MA5-14276 was used in immunohistochemistry to investigate the changes of gene expression in breast cancer cells under different status
|Konstantinovsky S,Smith Y,Zilber S,Tuft Stavnes H,Becker AM,Nesland JM,Reich R,Davidson B||Journal of oncology (2010:null)||2009|
Proteomic analysis of selected prognostic factors of breast cancer.
MA5-14276 was used in western blot to study the proteomics of selected prognostic factors in breast cancer
|Roberts K,Bhatia K,Stanton P,Lord R||Proteomics (4:784)||2004|