Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 4
p130Cas Antibody (PA5-120629) in IHC (P)
Immunohistochemistry analysis of p130Cas in paraffin-embedded mouse kidney. Samples were incubated with p130Cas Polyclonal antibody (Product # PA5-120629) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
PA5-120629
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 495-612 of human BCAR1 (NP_055382.2).
Conjugate
Form
Liquid
Concentration
0.52 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914201
Product Specific Information
Positive test controls include: HeLa, Mouse brain. The target is usually found in the following locations: Cell junction, Cytoplasm, focal adhesion.^M
^M
Immunogen sequence: EPQEPLVQDL QAAVAAVQSA VHELLEFARS AVGNAAHTSD RALHAKLSRQ LQKMEDVHQT LVAHGQALDA GRGGSGATLE DLDRLVACSR AVPEDAKQLA SFLHGNASLL FRRTKATA
Target Information
The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins. p130Cas has a unique structure that contains a Src homology (SH)-3 domain followed by multiple YXXP motifs and a proline-rich regionp130Cas is implicated in a variety of biological processes including cell adhesion, cell migration, growth factor stimulation, cytokine receptor engagement and bacterial infection. Physiological functions of p130Cas include cardiovascular development, actin filament assembly and Src-induced cell transformation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: BCA1; breast cancer anti-estrogen resistance 1; Breast cancer anti-estrogen resistance protein 1; Cas scaffolding protein family member 1; CRK-associated substrate; Crk-associated substrate p130Cas; OTTHUMP00000174941; p130 Cas; p130cas; p130Cas (pTyr410); p130Cas (pY410); p130Cas phospho; phospho p130Cas; v-crk-associated tyrosine kinase substrate
Gene Aliases: AI385681; BCAR1; CAS; CAS1; CASS1; CRKAS; P130CAS
UniProt ID: (Human) P56945, (Mouse) Q61140, (Rat) Q63767
Entrez Gene ID: (Human) 9564, (Mouse) 12927, (Rat) 25414
actin filament organization
cell adhesion
epidermal growth factor receptor signaling pathway
G-protein coupled receptor signaling pathway
integrin-mediated signaling pathway
insulin receptor signaling pathway
positive regulation of endothelial cell migration
cell migration
actin cytoskeleton organization
positive regulation of cell migration
cellular response to hepatocyte growth factor stimulus
platelet-derived growth factor receptor signaling pathway
vascular endothelial growth factor receptor signaling pathway
neurotrophin TRK receptor signaling pathway
hepatocyte growth factor receptor signaling pathway
antigen receptor-mediated signaling pathway
B cell receptor signaling pathway
cell chemotaxis
signal transduction
regulation of cell growth
cell proliferation
regulation of apoptotic process
T cell receptor signaling pathway
cell division
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support