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Invitrogen
p16INK4a-TAT Polyclonal Antibody, Biotin, PeproTech®
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p16INK4a-TAT Antibody (500-P284TBT-25UG) in WB
Western Blot: To detect Human p16-INK4a-TAT by Western Blot analysis, p16INK4a-TAT Polyclonal Antibody, Biotin (Product # 500-P284TBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human p16-INK4a-TAT is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P284TBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 18.0 kDa Recombinant Human p16-INK4a-TAT
Conjugate
Biotin
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930239
Product Specific Information
AA Sequence of recombinant protein: EPAAGSSMEP SADWLATAAA RGRVEEVRAL LEAGALPNAP NSYGRRPIQV MMMGSARVAE LLLLHGAEPN CADPATLTRP VHDAAREGFL DTLVVLHRAG ARLDVRDAWG RLPVDLAEEL GHRDVARYLR AAAGGTRGSN HARIDAAEGP SDIPDGYGR KKRRQRRR.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human p16-INK4a-TAT. Anti-Human p16-INK4a-TAT-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human p16-INK4a-TAT by sandwich ELISA (using 100 µL/well), a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody,in conjunction with PeproTech Polyclonal Anti-Human p16-INK4a-TAT (500-P284) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human p16-INK4a-TAT.
Western Blot: To detect Human p16-INK4a-TAT by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/mL.When used in conjunction with compatible development reagents, the detection limit for Recombinant Human p16-INK4a-TAT is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P284TBT-1MG will be provided as 2 x 500 µg
Target Information
p16-INK4a is a nuclear protein that regulates the cell cycle by inhibiting cyclin-dependent kinase-4 (CDK4) and CDK6. p16-INK4a inhibits CDK activity by binding to the CDK molecules in a manner that interferes with their ability to interact with cyclin D. This activity has the effect of suppressing tumor formation and growth, and of inducing replicative senescence in various normal cells, including stem cells. The expression of p16-INK4a steadily increases with age, and tends to accumulate in stem cell compartments. The deletion, rearrangement, or mutation of the p16-INK4a gene is frequently found in melanomas, as well as in certain other types of cancer. p16-INK4a and other transcription factors have been introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other nuclear proteins into primary, as well as transformed, cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alternative reading frame; ARF; CDK4 inhibitor p16-INK4; CDK4I; cell cycle negative regulator beta; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); MTS-1; Multiple tumor suppressor 1; p14ARF; p16-INK4; p16-INK4a; p16INK4a; Tumor suppressor ARF
Gene Aliases: ARF; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4; P16INK4A; P19; P19ARF; TP16
UniProt ID: (Human) P42771
Entrez Gene ID: (Human) 1029
G1/S transition of mitotic cell cycle
negative regulation of cell-matrix adhesion
cell cycle arrest
Ras protein signal transduction
negative regulation of cell proliferation
negative regulation of cell growth
negative regulation of NF-kappaB transcription factor activity
positive regulation of smooth muscle cell apoptotic process
senescence-associated heterochromatin focus assembly
negative regulation of phosphorylation
negative regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of transcription, DNA-templated
cellular senescence
replicative senescence
positive regulation of macrophage apoptotic process
positive regulation of cellular senescence
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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