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Immunofluorescence analysis of p21 was done on 70% confluent log phase U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with p21 Mouse Monoclonal Antibody (337000) at 2 µg - 4 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour® 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin (A12381). Panel d is a merged image showing nuclear localization of p21. Panel e shows no primary antibody. The images were captured at 20X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Full length recombinant human p21Cip1/WAF1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration.
33-7000 was used in immunocytochemistry to generate a model of stress-induced senescence and study the hepatocyte senescence associated secretory phenotype
|Irvine KM,Skoien R,Bokil NJ,Melino M,Thomas GP,Loo D,Gabrielli B,Hill MM,Sweet MJ,Clouston AD,Powell EE||World journal of gastroenterology (20:17851)||2014|
Targeting CDH17 suppresses tumor progression in gastric cancer by downregulating Wnt/β-catenin signaling.
33-7000 was used in western blot to report proof-of-principle studies targeting CDH7 for treating gastric cancer.
|Qiu HB,Zhang LY,Ren C,Zeng ZL,Wu WJ,Luo HY,Zhou ZW,Xu RH||PloS one (8:null)||2013|
MicroRNA-224 negatively regulates p21 expression during late neoplastic progression in inflammatory bowel disease.
33-7000 was used in western blot to investigate miR dysregulation in colonic specimens from patients with inflammatory bowel disease.
|Olaru AV,Yamanaka S,Vazquez C,Mori Y,Cheng Y,Abraham JM,Bayless TM,Harpaz N,Selaru FM,Meltzer SJ||Inflammatory bowel diseases (19:471)||2013|
|Human||Not Cited||Potent iron chelators increase the mRNA levels of the universal cyclin-dependent kinase inhibitor p21(CIP1/WAF1), but paradoxically inhibit its translation: a potential mechanism of cell cycle dysregulation.||Le NT,Richardson DR||Carcinogenesis (24:1045)||2003|
Genome-wide expression profiling of 8-chloroadenosine- and 8-chloro-cAMP-treated human neuroblastoma cells using radioactive human cDNA microarray.
33-7000 was used in western blot to analyze the cellular effects of 8-Cl-cAMP and 8-Cl-adenosine on human neuroblastoma cells using DNA arrays.
|Park GH,Choe J,Choo HJ,Park YG,Sohn J,Kim MK||Experimental & molecular medicine (34:184)||2002|
|Human||Not Cited||Clinical and immunohistochemical features associated with a response to bortezomib in patients with multiple myeloma.||Dawson MA,Opat SS,Taouk Y,Donovan M,Zammit M,Monaghan K,Horvath N,Roberts AW,Prince HM,Hertzberg M,McLean CA,Spencer A||Clinical cancer research : an official journal of the American Association for Cancer Research (15:714)||2009|
CAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CDNK1A; cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; p21CIP1; SDI1; WAF1; wild-type p53-activated fragment 1
CAP20; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; PIC1; SDI1; WAF1