|Tested species reactivity||Human|
|Host / Isotype||Rat / IgG1|
|Immunogen||Recombinant, human wildtype p21 (WAF1/CIP1/SDI1) protein.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-4800 detects p21 (WAF1/CIP1/SDI1) from human samples.
MA1-4800 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects 10 ng p21 (WAF1/CIP1/SDI1) protein. By ELISA this antibody detects 40 ng purified, recombinant p21 (WAF1/CIP1/SDI1) protein.
The MA1-4800 immunogen is recombinant, human wildtype p21 (WAF1/CIP1/SDI1) protein.
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.