|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant p21 protein.|
|Storage buffer||0.05M sodium phosphate, pH 7.5, with 0.2% gelatin|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||8-12 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||8-12 µg/ml|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-91045 detects cN RAS from rat, mouse, human samples.
MA1-91045 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), Western blot, immunoprecipitation applications.
The MA1-91045 immunogen is: Recombinant p21 protein.
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Histone Lysine Methylation in TGF-ß1 Mediated p21 Gene Expression in Rat Mesangial Cells.
MA1-91045 was used in western blot to study rat mesangial cells for histone lysine methylation in TGF-beta1 mediated p21 gene expression
|Guo Q,Li X,Han H,Li C,Liu S,Gao W,Sun G||BioMed research international (2016:null)||2016|