|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant p21WAF1 protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay-Dependent|
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.
Rapamycin induces pluripotent genes associated with avoidance of replicative senescence.
AHZ0422 was used in immunocytochemistry and western blot to suggest that suppression mTORC activity prevents replicative senescence without transformation of rodent cells.
|Pospelova TV,Bykova TV,Zubova SG,Katolikova NV,Yartzeva NM,Pospelov VA||Cell cycle (Georgetown, Tex.) (12:3841)||2013|
p21Waf1 is required for complete oncogenic transformation of mouse embryo fibroblasts by E1Aad5 and c-Ha-ras oncogenes.
AHZ0422 was used in western blot to determine the role of p21(Waf1) in the transformation process using mouse embryo fibroblasts.
|Romanov VS,Bardin AA,Zubova SG,Bykova TV,Pospelov VA,Pospelova TV||Biochimie (93:1408)||2011|
|Histone deacetylase inhibitor vorinostat suppresses the growth of uterine sarcomas in vitro and in vivo.||Hrzenjak A,Moinfar F,Kremser ML,Strohmeier B,Petru E,Zatloukal K,Denk H||Molecular cancer (9:-)||2010|
CAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CDNK1A; cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); cyclin-dependent kinase inhibitor 1A variant 1; cyclin-dependent kinase inhibitor 1A variant 2; DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; melanoma differentiation-associated protein; p21<WAF1/CIP1>; p21CIP1; SDI1; WAF1; wild-type p53-activated fragment 1
CAP20; CDKI; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; p21WAF; PIC1; SDI1; WAF1