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|Tested species reactivity||Human , Rat , Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant p21WAF1 protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Rapamycin induces pluripotent genes associated with avoidance of replicative senescence.
AHZ0422 was used in immunocytochemistry and western blot to suggest that suppression mTORC activity prevents replicative senescence without transformation of rodent cells.
|Pospelova TV,Bykova TV,Zubova SG,Katolikova NV,Yartzeva NM,Pospelov VA||Cell cycle (Georgetown, Tex.) (12:3841)||2013|
p21CIP1, SDI1, Cdkn1, MDA-6, mda6, CAP20, WAF1, Waf1, CDKN1, CIP1, P21, p21Cip1, p21WAF, CDKI
CDK-interacting protein 1, CDK-interaction protein 1, DNA synthesis inhibitor, cyclin-dependent kinase inhibitor 1, melanoma differentiation associated protein 6, wild-type p53-activated fragment 1, cyclin-dependent kinase inhibitor 1A variant 1, cyclin-dependent kinase inhibitor 1A variant 2, melanoma differentiation-associated protein, p21<WAF1/CIP1>, CAP20, CDKN1, CDNK1A, MDA-6, SDI1, WAF1, p21CIP1