Immunofluorescent analysis of p23 using Anti-p23 Monoclonal Antibody (JJ5) (Product# MA3-414) shows staining in Hela Cells. p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 (Product# MA3-414) at a dilution of 1:500 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
|Tested species reactivity||Chicken, Guinea pig, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Rabbit, Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human p23 protein.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/100|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-414 detects p23 from chicken, guinea pig, human, rat, mouse and rabbit samples.
MA3-414 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 23 kDa protein representing p23 from rabbit reticulocyte lysate. MA3-414 can be used to immunoprecipitate both free and complexed p23.
The MA3-414 antigen is recombinant human p23.
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP70 and HSP90), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 and FKBP54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP70, HSP90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex.
Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hsp70 cochaperones HspBP1 and BAG-1M differentially regulate steroid hormone receptor function.
MA3-414 was used in western blot to study the functional regulation of steroid hormone receptors by the Hsp70 co-chaperones HspBP1 and GAG-1M
|Knapp RT,Wong MJ,Kollmannsberger LK,Gassen NC,Kretzschmar A,Zschocke J,Hafner K,Young JC,Rein T||PloS one (9:null)||2014|
Hsp90 cochaperones p23 and FKBP4 physically interact with hAgo2 and activate RNA interference-mediated silencing in mammalian cells.
MA3-414 was used in western blot to study the interaction of p23 and FKBP4 with hAgo2 and their roles in the mechanism of mammalian cell RNAi silencing
|Pare JM,LaPointe P,Hobman TC||Molecular biology of the cell (24:2303)||2013|
|Not Applicable||Not Cited||
pRb controls estrogen receptor alpha protein stability and activity.
MA3-414 was used in western blot to investigate cross talk between the estrogen receptor and the retinoblastoma pathway
|Caligiuri I,Toffoli G,Giordano A,Rizzolio F||Oncotarget (4:875)||2013|
Hsp70 and Hsp90 multichaperone complexes sequentially regulate thiazide-sensitive cotransporter endoplasmic reticulum-associated degradation and biogenesis.
MA3-414 was used in western blot to study the role of Hsp70 and Hsp90 in the endoplasmic reticulum quality control of the thiazide-sensitive NaCl co-transporter
|Donnelly BF,Needham PG,Snyder AC,Roy A,Khadem S,Brodsky JL,Subramanya AR||The Journal of biological chemistry (288:13124)||2013|
p23 co-chaperone protects the aryl hydrocarbon receptor from degradation in mouse and human cell lines.
MA3-414 was used in western blot to study the role of p23 in protecting the aryl hydrocarbon receptor against degradation
|Nguyen PM,Wang D,Wang Y,Li Y,Uchizono JA,Chan WK||Biochemical pharmacology (84:838)||2012|
Type 1 inositol-1,4,5-trisphosphate receptor is a late substrate of caspases during apoptosis.
MA3-414 was used in western blot to study role of the type 1 inositol-1,4,5-trisphosphate receptor as an apoptotic substrate of caspases
|Elkoreh G,Blais V,Béliveau E,Guillemette G,Denault JB||Journal of cellular biochemistry (113:2775)||2012|
Caspase-1 promiscuity is counterbalanced by rapid inactivation of processed enzyme.
MA3-414 was used in western blot to investigate the substrate specificity of caspase-1
|Walsh JG,Logue SE,Lüthi AU,Martin SJ||The Journal of biological chemistry (286:32513)||2011|
Molecular determinants involved in activation of caspase 7.
MA3-414 was used in western blot to investigate the caspase activation pathway during apoptosis
|Boucher D,Blais V,Drag M,Denault JB||Bioscience reports (31:283)||2011|
Calpain and caspase processing of caspase-12 contribute to the ER stress-induced cell death pathway in differentiated PC12 cells.
MA3-414 was used in western blot to investigate the involvement of calpain and caspase 12 in the cell death induced by endoplasmic reticulum stress
|Martinez JA,Zhang Z,Svetlov SI,Hayes RL,Wang KK,Larner SF||Apoptosis : an international journal on programmed cell death (15:1480)||2010|
ATP binding to Hsp90 is sufficient for effective chaperoning of p53 protein.
MA3-414 was used in western blot to study the mechanistic role of ATP in the Hsp90-p53 interaction.
|Walerych D,Gutkowska M,Klejman MP,Wawrzynow B,Tracz Z,Wiech M,Zylicz M,Zylicz A||The Journal of biological chemistry (285:32020)||2010|
Glucose deprivation induces an atypical form of apoptosis mediated by caspase-8 in Bax-, Bak-deficient cells.
MA3-414 was used in western blot to investigate the mechanism of apoptosis induced by glucose deprivation in Bax- and Bak-deficient cells
|Caro-Maldonado A,Tait SW,Ramírez-Peinado S,Ricci JE,Fabregat I,Green DR,Muñoz-Pinedo C||Cell death and differentiation (17:1335)||2010|
The hsp90-FKBP52 complex links the mineralocorticoid receptor to motor proteins and persists bound to the receptor in early nuclear events.
MA3-414 was used in western blot to investigate the role of the heat shock protein 90-FKBP52 complex in the nucleus
|Galigniana MD,Erlejman AG,Monte M,Gomez-Sanchez C,Piwien-Pilipuk G||Molecular and cellular biology (30:1285)||2010|
Ordering of caspases in cells undergoing apoptosis by the intrinsic pathway.
MA3-414 was used in western blot to investigate the hierarchical ordering of caspases in a more relevant physiological scenario
|Inoue S,Browne G,Melino G,Cohen GM||Cell death and differentiation (16:1053)||2009|
17-DMAG ameliorates polyglutamine-mediated motor neuron degeneration through well-preserved proteasome function in an SBMA model mouse.
MA3-414 was used in western blot to study the effect of 17-(dimethylaminoethylamino)-17-demethoxygeldanamycin on polyglutamine-mediated motor neuron degeneration.
|Tokui K,Adachi H,Waza M,Katsuno M,Minamiyama M,Doi H,Tanaka K,Hamazaki J,Murata S,Tanaka F,Sobue G||Human molecular genetics (18:898)||2009|
Multiple 40-kDa heat-shock protein chaperones function in Tom70-dependent mitochondrial import.
MA3-414 was used in western blot to investigate the roles of multiple 40-kDa heat-shock protein chaperones during the Tom70-dependent mitochondrial import.
|Bhangoo MK,Tzankov S,Fan AC,Dejgaard K,Thomas DY,Young JC||Molecular biology of the cell (18:3414)||2007|
Cytosolic prostaglandin E2 synthase (cPGES) expression is decreased in discrete cortical regions in psychiatric disease.
MA3-414 was used in western blot to investigate the change of expression of cytosolic prostaglandin E2 synthase in psychiatric patients
|Maida ME,Hurley SD,Daeschner JA,Moore AH,O'Banion MK||Brain research (1103:164)||2006|
17-AAG, an Hsp90 inhibitor, ameliorates polyglutamine-mediated motor neuron degeneration.
MA3-414 was used in western blot to investigate the role of 17-AAG in curing spinal and bulbar muscular atrophy as well as degradation of polyglutamine-expanded mutant AR
|Waza M,Adachi H,Katsuno M,Minamiyama M,Sang C,Tanaka F,Inukai A,Doyu M,Sobue G||Nature medicine (11:1088)||2005|
Microsomal prostaglandin E synthase-1 is overexpressed in inflammatory bowel disease. Evidence for involvement of the transcription factor Egr-1.
MA3-414 was used in western blot to study the effect of Egr-1-mediated transcription activation on mPGES-1 expression in inflammatory bowel disease .
|Subbaramaiah K,Yoshimatsu K,Scherl E,Das KM,Glazier KD,Golijanin D,Soslow RA,Tanabe T,Naraba H,Dannenberg AJ||The Journal of biological chemistry (279:12647)||2004|
High levels of Hsp90 cochaperone p23 promote tumor progression and poor prognosis in breast cancer by increasing lymph node metastases and drug resistance.
MA3-414 was used in immunohistochemistry to investigate the mechanism of Hsp90 cochaperone p23 in tumor progression and poor prognosis in breast cancer
|Simpson NE,Lambert WM,Watkins R,Giashuddin S,Huang SJ,Oxelmark E,Arju R,Hochman T,Goldberg JD,Schneider RJ,Reiz LF,Soares FA,Logan SK,Garabedian MJ||Cancer research (70:8446)||2010|
Expression of Hsp90 chaperone [corrected] proteins in human tumor tissue.
MA3-414 was used in immunohistochemistry to study the expression profiling of chaperone proteins in human tumor tissue samples
|McDowell CL,Bryan Sutton R,Obermann WM||International journal of biological macromolecules (45:310)||2009|
Expression and localization of adipophilin and perilipin in human fetal membranes: association with lipid bodies and enzymes involved in prostaglandin synthesis.
MA3-414 was used in immunohistochemistry to study the expression of adipophilin and perilipin in human fetal membranes at preterm and term.
|Meadows JW,Pitzer B,Brockman DE,Myatt L||The Journal of clinical endocrinology and metabolism (90:2344)||2005|
Nuclear import of the glucocorticoid receptor-hsp90 complex through the nuclear pore complex is mediated by its interaction with Nup62 and importin beta.
MA3-414 was used in immunoprecipitation to study the mechanism of nuclear import of the glucocorticoid receptor-hsp90 complex through the nuclear pore complex
|Echeverría PC,Mazaira G,Erlejman A,Gomez-Sanchez C,Piwien Pilipuk G,Galigniana MD||Molecular and cellular biology (29:4788)||2009|
Hsp90 regulates the Fanconi anemia DNA damage response pathway.
MA3-414 was used in immunoprecipitation to study the role of Hsp90 in regulating the Fanconi anemia DNA damage response pathway
|Oda T,Hayano T,Miyaso H,Takahashi N,Yamashita T||Blood (109:5016)||2007|
Progesterone receptor deficient in chromatin binding has an altered cellular state.
MA3-414 was used in immunoprecipitation to analyze the altered cellular processing by comparing the composition and distribution of tPR and sPR complexes
|Botos J,Xian W,Smith DF,Smith CL||The Journal of biological chemistry (279:15231)||2004|
The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties.
MA3-414 was used in immunoprecipitation to investigate the functional properties HSP90-related protein TRAP1 in mitochondria
|Felts SJ,Owen BA,Nguyen P,Trepel J,Donner DB,Toft DO||The Journal of biological chemistry (275:3305)||2000|
Active participation of Hsp90 in the biogenesis of the trimeric reovirus cell attachment protein sigma1.
MA3-414 was used in immunoprecipitation to demonstrate that chaperones play an important role in the regulation of general protein folding and of steroid receptor activation
|Gilmore R,Coffey MC,Lee PW||The Journal of biological chemistry (273:15227)||1998|
FK506 requires stimulation of the extracellular signal-regulated kinase 1/2 and the steroid receptor chaperone protein p23 for neurite elongation.
MA3-414 was used in immunocytochemistry to study the role of the ERK and steroid receptor chaperone protein p23 in the neurite elongation activity of FK506
|Gold BG,Zhong YP||Neuro-Signals (13:122)||2004|
Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes.
MA3-414 was used in immunocytochemistry to investigate the role of chaperones and proteasomes during glucocorticoid receptor exchange.
|Stavreva DA,Müller WG,Hager GL,Smith CL,McNally JG||Molecular and cellular biology (24:2682)||2004|